{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["25(4)"],"submitter":["Harada Y"],"pubmed_abstract":["Intestinal intraepithelial lymphocytes (IELs), the first line of defense against microbial and dietary antigens, are classified as natural or induced based on their origin and receptor expression. Induced CD4<sup>+</sup>CD8αα<sup>+</sup>TCRβ<sup>+</sup> T cells (double positive, DP<sub>IELs</sub>) originated from CD4<sup>+</sup>CD8α<sup>-</sup>TCRβ<sup>+</sup> T cells (single positive, SP<sub>IELs</sub>) increase with aging. However, the metabolic requirements and the metabolic-related genes in IEL development remain unclear. We determined that the intraepithelial compartment is hypoxic in the presence of microbes and DP<sub>IELs</sub> increased more than natural IELs in this location. Moreover, DP<sub>IELs</sub> consumed less oxygen and glucose and exhibited unique alterations in mitochondria. Using inhibitors and genetically modified mice, we revealed that DP<sub>IELs</sub> adapt to their surrounding oxygen-deprived environment in peripheral tissues by modulating specific genes, including hypoxia-inducible factor, mammalian target of rapamycin complexes (mTORC), phosphorylated ribosomal protein S6 (pS6), and other glycolytic factors. Our findings provide valuable insight into the metabolic properties of IELs."],"journal":["iScience"],"pagination":["104021"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8933710"],"repository":["biostudies-literature"],"pubmed_title":["Intracellular metabolic adaptation of intraepithelial CD4<sup>+</sup>CD8αα<sup>+</sup> T lymphocytes."],"pmcid":["PMC8933710"],"pubmed_authors":["Harada Y","Sujino T","Hirao A","Kanai T","Ogata H","Hosoe N","Kubota Y","Tanemoto S","Yoshimatsu Y","Nomura E","Miyamoto K","Umeda S","Mikami Y","Nakamoto N","Takabayashi K","Ikenoue T","Ono K"],"additional_accession":[]},"is_claimable":false,"name":"Intracellular metabolic adaptation of intraepithelial CD4<sup>+</sup>CD8αα<sup>+</sup> T lymphocytes.","description":"Intestinal intraepithelial lymphocytes (IELs), the first line of defense against microbial and dietary antigens, are classified as natural or induced based on their origin and receptor expression. Induced CD4<sup>+</sup>CD8αα<sup>+</sup>TCRβ<sup>+</sup> T cells (double positive, DP<sub>IELs</sub>) originated from CD4<sup>+</sup>CD8α<sup>-</sup>TCRβ<sup>+</sup> T cells (single positive, SP<sub>IELs</sub>) increase with aging. However, the metabolic requirements and the metabolic-related genes in IEL development remain unclear. We determined that the intraepithelial compartment is hypoxic in the presence of microbes and DP<sub>IELs</sub> increased more than natural IELs in this location. Moreover, DP<sub>IELs</sub> consumed less oxygen and glucose and exhibited unique alterations in mitochondria. Using inhibitors and genetically modified mice, we revealed that DP<sub>IELs</sub> adapt to their surrounding oxygen-deprived environment in peripheral tissues by modulating specific genes, including hypoxia-inducible factor, mammalian target of rapamycin complexes (mTORC), phosphorylated ribosomal protein S6 (pS6), and other glycolytic factors. Our findings provide valuable insight into the metabolic properties of IELs.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-05T14:43:32.242Z","creation":"2025-04-05T14:43:32.242Z"},"accession":"S-EPMC8933710","cross_references":{"pubmed":["35313689"],"doi":["10.1016/j.isci.2022.104021"]}}