{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Speidel A"],"funding":["Eberhard Karls Universität Tübingen"],"pagination":["104014"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8933718"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(4)"],"pubmed_abstract":["<i>Trypanosoma brucei</i> is the causative agent of human African trypanosomiasis. The parasite transmigrates from blood vessels across the choroid plexus epithelium to enter the central nervous system, a process that leads to the manifestation of second stage sleeping sickness. Using an <i>in vitro</i> model of the blood-cerebrospinal fluid barrier, we investigated the mechanism of the transmigration process. For this, a monolayer of human choroid plexus papilloma cells was cultivated on a permeable membrane that mimics the basal lamina underlying the choroid plexus epithelial cells. Plexus cells polarize and interconnect forming tight junctions. Deploying different <i>T. brucei brucei</i> strains, we observed that geometry and motility are important for tissue invasion. Using fluorescent microscopy, the parasite's moving was visualized between plexus epithelial cells. The presented model provides a simple tool to screen trypanosome libraries for their ability to infect cerebrospinal fluid or to test the impact of chemical substances on transmigration."],"journal":["iScience"],"pubmed_title":["Transmigration of <i>Trypanosoma brucei</i> across an <i>in vitro</i> blood-cerebrospinal fluid barrier."],"pmcid":["PMC8933718"],"funding_grant_id":["2465-0-0"],"pubmed_authors":["Theile M","Duszenko M","Schwerk C","Schroten H","Pfeiffer L","Mogk S","Figarella K","Herrmann A","Speidel A","Ishikawa H"],"additional_accession":[]},"is_claimable":false,"name":"Transmigration of <i>Trypanosoma brucei</i> across an <i>in vitro</i> blood-cerebrospinal fluid barrier.","description":"<i>Trypanosoma brucei</i> is the causative agent of human African trypanosomiasis. The parasite transmigrates from blood vessels across the choroid plexus epithelium to enter the central nervous system, a process that leads to the manifestation of second stage sleeping sickness. Using an <i>in vitro</i> model of the blood-cerebrospinal fluid barrier, we investigated the mechanism of the transmigration process. For this, a monolayer of human choroid plexus papilloma cells was cultivated on a permeable membrane that mimics the basal lamina underlying the choroid plexus epithelial cells. Plexus cells polarize and interconnect forming tight junctions. Deploying different <i>T. brucei brucei</i> strains, we observed that geometry and motility are important for tissue invasion. Using fluorescent microscopy, the parasite's moving was visualized between plexus epithelial cells. The presented model provides a simple tool to screen trypanosome libraries for their ability to infect cerebrospinal fluid or to test the impact of chemical substances on transmigration.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-18T20:23:31.967Z","creation":"2025-04-07T08:19:22.807Z"},"accession":"S-EPMC8933718","cross_references":{"pubmed":["35313698"],"doi":["10.1016/j.isci.2022.104014"]}}