biostudies-literatureZhang YAnhui ProvinceNational Natural Science Foundation of Chinae13086https://www.ebi.ac.uk/biostudies/studies/S-EPMC8934043biostudies-literatureUnknown10Cystathionine γ-synthase (<i>CGS</i>), S-adenosyl-L-homocysteine hydrolase (<i>SAHH</i>), and S-adenosy-L-methionine synthetase (<i>SAMS</i>) play an important role in the regulation of plant growth, development, and secondary metabolism. In this study, a total of 6 <i>CGS</i>, 6 <i>SAHH</i>, and 28 <i>SAMS</i> genes were identified from five Rosaceae species (<i>Pyrus bretschneideri</i>, <i>Prunus persica</i>, <i>Prunus mume</i>, <i>Fragaria vesca</i>, and <i>Malus domestica</i>). The evolutionary relationship and microsynteny analysis in five Rosaceae species revealed that duplicated regions were conserved between three gene families (<i>CGS</i>, <i>SAHH</i>, <i>SAMS</i>). Moreover, the chromosomal locations, gene structures, conserved motifs, <i>cis</i>-elements, physicochemical properties, and Ka/Ks analysis were performed by using numerous bioinformatics tools. The expression of different organs showed that the <i>CGS</i>, <i>SAHH</i> and <i>SAMS</i> genes of pear have relatively high expression patterns in flowers and stems, except for <i>PbCGS1</i>. RNA-seq and qRT-PCR combined analysis showed that <i>PbSAMS1</i> may be involved in the regulation of pear stone cell development. In summary, this study provides the basic information of <i>CGS</i>, <i>SAHH</i> and <i>SAMS</i> genes in five Rosaceae species, further revealing the expression patterns in the pear fruit, which provides the theoretical basis for the regulation of pear stone cells.PeerJWhole-genome analysis of <i>CGS</i>, <i>SAHH</i>, <i>SAMS</i> gene families in five Rosaceae species and their expression analysis in <i>Pyrus bretschneideri</i>.PMC8934043YJS2021023131640068Wang MLi GLi DZhang YFeng XHan WCai YWang XManzoor MAWang HChen TfalseWhole-genome analysis of <i>CGS</i>, <i>SAHH</i>, <i>SAMS</i> gene families in five Rosaceae species and their expression analysis in <i>Pyrus bretschneideri</i>.Cystathionine γ-synthase (<i>CGS</i>), S-adenosyl-L-homocysteine hydrolase (<i>SAHH</i>), and S-adenosy-L-methionine synthetase (<i>SAMS</i>) play an important role in the regulation of plant growth, development, and secondary metabolism. In this study, a total of 6 <i>CGS</i>, 6 <i>SAHH</i>, and 28 <i>SAMS</i> genes were identified from five Rosaceae species (<i>Pyrus bretschneideri</i>, <i>Prunus persica</i>, <i>Prunus mume</i>, <i>Fragaria vesca</i>, and <i>Malus domestica</i>). The evolutionary relationship and microsynteny analysis in five Rosaceae species revealed that duplicated regions were conserved between three gene families (<i>CGS</i>, <i>SAHH</i>, <i>SAMS</i>). Moreover, the chromosomal locations, gene structures, conserved motifs, <i>cis</i>-elements, physicochemical properties, and Ka/Ks analysis were performed by using numerous bioinformatics tools. The expression of different organs showed that the <i>CGS</i>, <i>SAHH</i> and <i>SAMS</i> genes of pear have relatively high expression patterns in flowers and stems, except for <i>PbCGS1</i>. RNA-seq and qRT-PCR combined analysis showed that <i>PbSAMS1</i> may be involved in the regulation of pear stone cell development. In summary, this study provides the basic information of <i>CGS</i>, <i>SAHH</i> and <i>SAMS</i> genes in five Rosaceae species, further revealing the expression patterns in the pear fruit, which provides the theoretical basis for the regulation of pear stone cells.2022-01-01T00:00:00Z20222026-05-30T19:51:58.195Z2025-05-29T22:20:37.509ZS-EPMC89340433531352610.7717/peerj.13086