biostudies-literatureUnknown63(3)He X<h4>Purpose</h4>To investigate microenvironment changes of the lacrimal gland after obstruction of lacrimal gland ducts.<h4>Methods</h4>The ducts of rat exorbital lacrimal gland were ligated by sutures for different durations. After that, the sutures in some animals were released, and they were observed for 21 days to evaluate the recovery of the lacrimal gland. Slit lamp and tear secretion test was performed to evaluate ocular surface and lacrimal gland function. The lacrimal gland and cornea were harvested and processed for hematoxylin and eosin staining, oil red O staining, LipidTOX staining, Masson staining, quantitative real time polymerase chain reaction, and immunofluorescence staining.<h4>Results</h4>After the lacrimal gland ducts were blocked, tear secretion and the weight of the Investigative ophthalmology & visual science14https://www.ebi.ac.uk/biostudies/studies/S-EPMC8934564biostudies-literatureLacrimal Gland Microenvironment Changes After Obstruction of Lacrimal Gland Ducts.PMC8934564Li WLiu ZWang SHe XHe HSun HWu YZhuang JShi YWu HfalseLacrimal Gland Microenvironment Changes After Obstruction of Lacrimal Gland Ducts.<h4>Purpose</h4>To investigate microenvironment changes of the lacrimal gland after obstruction of lacrimal gland ducts.<h4>Methods</h4>The ducts of rat exorbital lacrimal gland were ligated by sutures for different durations. After that, the sutures in some animals were released, and they were observed for 21 days to evaluate the recovery of the lacrimal gland. Slit lamp and tear secretion test was performed to evaluate ocular surface and lacrimal gland function. The lacrimal gland and cornea were harvested and processed for hematoxylin and eosin staining, oil red O staining, LipidTOX staining, Masson staining, quantitative real time polymerase chain reaction, and immunofluorescence staining.<h4>Results</h4>After the lacrimal gland ducts were blocked, tear secretion and the weight of the 2022-01-01T00:00:00Z2022 Mar2025-04-04T20:38:22.525Z2025-04-04T20:38:22.525ZS-EPMC89345643528984510.1167/iovs.63.3.14