{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(4)"],"submitter":["Wang S"],"pubmed_abstract":["The present study aims to increase pyruvate production by engineering <i>Yarrowia lipolytica</i> through modifying the glycerol metabolic pathway. Results: Wild-type <i>Yarrowia lipolytica</i> (Po1d) was engineered to produce six different strains, namely ZS099 (by over-expressing PYK1), ZS100 (by deleting DGA2), ZS101 (by over-expressing DAK1, DAK2, and GCY1), ZS102 (by over-expressing GUT1 and GUT2), ZS103 (by over-expressing GUT1) and ZSGP (by over-expressing POS5 and deleting GPD2). Production of pyruvate from engineered and control strains was determined using high-performance liquid chromatography (HPLC). Subsequently, the fermentation conditions for producing pyruvate were optimized, including the amount of initial inoculation, the addition of calcium carbonate (CaCO<sub>3</sub>), thiamine and glycerol. Finally, for scaled-up purposes, a 20-L fermentor was used. It was observed that pyruvate production increased by 136% (8.55 g/L) in ZSGP strain compared to control (3.62 g/L). Furthermore, pyruvate production by ZSGP reached up to 110.4 g/L in 96 h in the scaled-up process. We conclude that ZSGP strain of <i>Y. lipolytica</i> can be effectively used for pyruvate production at the industrial level.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s13205-022-03158-7."],"journal":["3 Biotech"],"pagination":["98"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8934898"],"repository":["biostudies-literature"],"pubmed_title":["Engineering of <i>Yarrowia lipolytica</i> for producing pyruvate from glycerol."],"pmcid":["PMC8934898"],"pubmed_authors":["Zheng J","Yang Y","Liu M","Wang S","Zhang Y","Xu S","Yu K","Yuan W","Sun J"],"additional_accession":[]},"is_claimable":false,"name":"Engineering of <i>Yarrowia lipolytica</i> for producing pyruvate from glycerol.","description":"The present study aims to increase pyruvate production by engineering <i>Yarrowia lipolytica</i> through modifying the glycerol metabolic pathway. Results: Wild-type <i>Yarrowia lipolytica</i> (Po1d) was engineered to produce six different strains, namely ZS099 (by over-expressing PYK1), ZS100 (by deleting DGA2), ZS101 (by over-expressing DAK1, DAK2, and GCY1), ZS102 (by over-expressing GUT1 and GUT2), ZS103 (by over-expressing GUT1) and ZSGP (by over-expressing POS5 and deleting GPD2). Production of pyruvate from engineered and control strains was determined using high-performance liquid chromatography (HPLC). Subsequently, the fermentation conditions for producing pyruvate were optimized, including the amount of initial inoculation, the addition of calcium carbonate (CaCO<sub>3</sub>), thiamine and glycerol. Finally, for scaled-up purposes, a 20-L fermentor was used. It was observed that pyruvate production increased by 136% (8.55 g/L) in ZSGP strain compared to control (3.62 g/L). Furthermore, pyruvate production by ZSGP reached up to 110.4 g/L in 96 h in the scaled-up process. We conclude that ZSGP strain of <i>Y. lipolytica</i> can be effectively used for pyruvate production at the industrial level.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s13205-022-03158-7.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-21T22:34:35.234Z","creation":"2025-04-05T18:49:18.128Z"},"accession":"S-EPMC8934898","cross_references":{"pubmed":["35463047"],"doi":["10.1007/s13205-022-03158-7"]}}