biostudies-literatureUnknown12(4)Wang SThe present study aims to increase pyruvate production by engineering <i>Yarrowia lipolytica</i> through modifying the glycerol metabolic pathway. Results: Wild-type <i>Yarrowia lipolytica</i> (Po1d) was engineered to produce six different strains, namely ZS099 (by over-expressing PYK1), ZS100 (by deleting DGA2), ZS101 (by over-expressing DAK1, DAK2, and GCY1), ZS102 (by over-expressing GUT1 and GUT2), ZS103 (by over-expressing GUT1) and ZSGP (by over-expressing POS5 and deleting GPD2). Production of pyruvate from engineered and control strains was determined using high-performance liquid chromatography (HPLC). Subsequently, the fermentation conditions for producing pyruvate were optimized, including the amount of initial inoculation, the addition of calcium carbonate (CaCO₃), thiamine and glycerol. Finally, for scaled-up purposes, a 20-L fermentor was used. It was observed that pyruvate production increased by 136% (8.55 g/L) in ZSGP strain compared to control (3.62 g/L). Furthermore, pyruvate production by ZSGP reached up to 110.4 g/L in 96 h in the scaled-up process. We conclude that ZSGP strain of <i>Y. lipolytica</i> can be effectively used for pyruvate production at the industrial level.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s13205-022-03158-7.3 Biotech98https://www.ebi.ac.uk/biostudies/studies/S-EPMC8934898biostudies-literatureEngineering of <i>Yarrowia lipolytica</i> for producing pyruvate from glycerol.PMC8934898Zheng JYang YLiu MWang SZhang YXu SYu KYuan WSun JfalseEngineering of <i>Yarrowia lipolytica</i> for producing pyruvate from glycerol.The present study aims to increase pyruvate production by engineering <i>Yarrowia lipolytica</i> through modifying the glycerol metabolic pathway. Results: Wild-type <i>Yarrowia lipolytica</i> (Po1d) was engineered to produce six different strains, namely ZS099 (by over-expressing PYK1), ZS100 (by deleting DGA2), ZS101 (by over-expressing DAK1, DAK2, and GCY1), ZS102 (by over-expressing GUT1 and GUT2), ZS103 (by over-expressing GUT1) and ZSGP (by over-expressing POS5 and deleting GPD2). Production of pyruvate from engineered and control strains was determined using high-performance liquid chromatography (HPLC). Subsequently, the fermentation conditions for producing pyruvate were optimized, including the amount of initial inoculation, the addition of calcium carbonate (CaCO₃), thiamine and glycerol. Finally, for scaled-up purposes, a 20-L fermentor was used. It was observed that pyruvate production increased by 136% (8.55 g/L) in ZSGP strain compared to control (3.62 g/L). Furthermore, pyruvate production by ZSGP reached up to 110.4 g/L in 96 h in the scaled-up process. We conclude that ZSGP strain of <i>Y. lipolytica</i> can be effectively used for pyruvate production at the industrial level.<h4>Supplementary information</h4>The online version contains supplementary material available at 10.1007/s13205-022-03158-7.2022-01-01T00:00:00Z2022 Apr2025-04-21T22:34:35.234Z2025-04-05T18:49:18.128ZS-EPMC89348983546304710.1007/s13205-022-03158-7