biostudies-literatureUnknown13Zhao YBased on the genome and small-RNA sequencing of pomegranate, miRNA167 and three target genes <i>PgARF6</i> were identified in "Taishanhong" genome. Three <i>PgARF6</i> genes and their corresponding protein sequences, expression patterns in pomegranate flower development and under exogenous hormones treatments were systematically analyzed in this paper. We found that PgARF6s are nuclear proteins with conserved structures. However, PgARF6s had different protein structures and expression profiles in pomegranate flower development. At the critical stages of pomegranate ovule sterility (8.1-14.0 mm), the expression levels of <i>PgARF6s</i> in bisexual flowers were lower than those in functional male flowers. Interestingly, <i>PgARF6c</i> expression level was significantly higher than <i>PgARF6aFrontiers in plant science833747https://www.ebi.ac.uk/biostudies/studies/S-EPMC8937018biostudies-literature<i>ARF6s</i> Identification and Function Analysis Provide Insights Into Flower Development of <i>Punica granatum</i> L.PMC8937018Yan MRen YWang YZhao YYuan ZZhao Xfalse<i>ARF6s</i> Identification and Function Analysis Provide Insights Into Flower Development of <i>Punica granatum</i> L.Based on the genome and small-RNA sequencing of pomegranate, miRNA167 and three target genes <i>PgARF6</i> were identified in "Taishanhong" genome. Three <i>PgARF6</i> genes and their corresponding protein sequences, expression patterns in pomegranate flower development and under exogenous hormones treatments were systematically analyzed in this paper. We found that PgARF6s are nuclear proteins with conserved structures. However, PgARF6s had different protein structures and expression profiles in pomegranate flower development. At the critical stages of pomegranate ovule sterility (8.1-14.0 mm), the expression levels of <i>PgARF6s</i> in bisexual flowers were lower than those in functional male flowers. Interestingly, <i>PgARF6c</i> expression level was significantly higher than <i>PgARF6a2022-01-01T00:00:00Z20222026-05-30T23:03:05.268Z2026-04-08T07:43:03.937ZS-EPMC89370183532144510.3389/fpls.2022.833747