{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jacob P"],"funding":["Lineberger Comprehensive Cancer Center, University of North Carolina","National Key Laboratory of Plant Molecular Genetics","Deutsche Forschungsgemeinschaft","Howard Hughes Medical Institute","National Research Foundation of Korea","National Institutes of Health","Reinhard Frank Stiftung","University of Tübingen core funding","NIGMS NIH HHS","National Science Foundation"],"pagination":["420-425"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8939002"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["373(6553)"],"pubmed_abstract":["Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In <i>Arabidopsis</i>, a subfamily of \"helper\" NLRs is required by many \"sensor\" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta, and conferred cytoplasmic calcium ion (Ca<sup>2+</sup>) influx in plant and human cells. NRG1.1-dependent Ca<sup>2+</sup> influx and cell death were sensitive to Ca<sup>2+</sup> channel blockers and were suppressed by mutations affecting oligomerization or plasma membrane enrichment. Ca<sup>2+</sup> influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another helper NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrated that <i>Arabidopsis</i> helper NLRs form Ca<sup>2+</sup>-permeable cation channels to directly regulate cytoplasmic Ca<sup>2+</sup> levels and consequent cell death. Thus, helper NLRs transduce cell death signals directly."],"journal":["Science (New York, N.Y.)"],"pubmed_title":["Plant \"helper\" immune receptors are Ca<sup>2+</sup>-permeable nonselective cation channels."],"pmcid":["PMC8939002"],"funding_grant_id":["R01 GM137286","2014R1A6A3A03058629","GM137286 and GM135218","R01 GM135218","P30 CA016086","XDB27040214","IOS-1457257","SFB/CRC1101 - project D09","IOS-1758400"],"pubmed_authors":["Wan L","Lietzan AD","Sunil S","Walton WG","Wu F","Furzer OJ","Dangl JL","Redinbo MR","Jacob P","Kempthorn K","El-Kasmi F","Pei ZM","Chi Y","Kim NH"],"additional_accession":[]},"is_claimable":false,"name":"Plant \"helper\" immune receptors are Ca<sup>2+</sup>-permeable nonselective cation channels.","description":"Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In <i>Arabidopsis</i>, a subfamily of \"helper\" NLRs is required by many \"sensor\" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta, and conferred cytoplasmic calcium ion (Ca<sup>2+</sup>) influx in plant and human cells. NRG1.1-dependent Ca<sup>2+</sup> influx and cell death were sensitive to Ca<sup>2+</sup> channel blockers and were suppressed by mutations affecting oligomerization or plasma membrane enrichment. Ca<sup>2+</sup> influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another helper NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrated that <i>Arabidopsis</i> helper NLRs form Ca<sup>2+</sup>-permeable cation channels to directly regulate cytoplasmic Ca<sup>2+</sup> levels and consequent cell death. Thus, helper NLRs transduce cell death signals directly.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul","modification":"2025-04-04T23:33:06.545Z","creation":"2025-04-04T23:33:06.545Z"},"accession":"S-EPMC8939002","cross_references":{"pubmed":["34140391"],"doi":["10.1126/science.abg7917"]}}