{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Patteson JB"],"funding":["NICHD NIH HHS","NIGMS NIH HHS"],"pagination":["1005-1009"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8939262"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["374(6570)"],"pubmed_abstract":["Metal-binding natural products contribute to metal acquisition and bacterial virulence, but their roles in metal stress response are underexplored. We show that a five-enzyme pathway in <i>Pseudomonas aeruginosa</i> synthesizes a small-molecule copper complex, fluopsin C, in response to elevated copper concentrations. Fluopsin C is a broad-spectrum antibiotic that contains a copper ion chelated by two minimal thiohydroxamates. Biosynthesis of the thiohydroxamate begins with cysteine and requires two lyases, two iron-dependent enzymes, and a methyltransferase. The iron-dependent enzymes remove the carboxyl group and the α carbon from cysteine through decarboxylation, N-hydroxylation, and methylene excision. Conservation of the pathway in <i>P. aeruginosa</i> and other bacteria suggests a common role for fluopsin C in the copper stress response, which involves fusing copper into an antibiotic against other microbes."],"journal":["Science (New York, N.Y.)"],"pubmed_title":["Biosynthesis of fluopsin C, a copper-containing antibiotic from <i>Pseudomonas aeruginosa</i>."],"pmcid":["PMC8939262"],"funding_grant_id":["R35 GM126961","T32 GM008570","DP2 HD094657"],"pubmed_authors":["Britt RD","Tao L","Bryant LH","Simke WC","Li B","Putz AT","Patteson JB"],"additional_accession":[]},"is_claimable":false,"name":"Biosynthesis of fluopsin C, a copper-containing antibiotic from <i>Pseudomonas aeruginosa</i>.","description":"Metal-binding natural products contribute to metal acquisition and bacterial virulence, but their roles in metal stress response are underexplored. We show that a five-enzyme pathway in <i>Pseudomonas aeruginosa</i> synthesizes a small-molecule copper complex, fluopsin C, in response to elevated copper concentrations. Fluopsin C is a broad-spectrum antibiotic that contains a copper ion chelated by two minimal thiohydroxamates. Biosynthesis of the thiohydroxamate begins with cysteine and requires two lyases, two iron-dependent enzymes, and a methyltransferase. The iron-dependent enzymes remove the carboxyl group and the α carbon from cysteine through decarboxylation, N-hydroxylation, and methylene excision. Conservation of the pathway in <i>P. aeruginosa</i> and other bacteria suggests a common role for fluopsin C in the copper stress response, which involves fusing copper into an antibiotic against other microbes.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2024-11-09T22:27:45.109Z","creation":"2024-11-09T22:27:45.109Z"},"accession":"S-EPMC8939262","cross_references":{"pubmed":["34793213"],"doi":["10.1126/science.abj6749"]}}