{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Heffernan JM"],"funding":["NICHD NIH HHS","Ben and Catherine Ivy Foundation","NINDS NIH HHS","National Institutes of Health","Barrow Neurological Foundation"],"pagination":["819-833"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8939461"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(3)"],"pubmed_abstract":["Glioblastoma (GBM) brain tumors contain a subpopulation of self-renewing multipotent Glioblastoma stem-like cells (GSCs) that are believed to drive the near inevitable recurrence of GBM. We previously engineered temperature responsive scaffolds based on the polymer poly(<i>N</i>-isopropylacrylamide-<i>co</i>-Jeffamine M-1000 acrylamide) (PNJ) for the purpose of enriching GSCs <i>in vitro</i> from patient-derived samples. Here, we used PNJ scaffolds to study microenvironmental regulation of self-renewal and radiation response in patient-derived GSCs representing classical and proneural subtypes. GSC self-renewal was regulated by the composition of PNJ scaffolds and varied with cell type. PNJ scaffolds protected against radiation-induced cell death, particularly in conditions that also promoted GSC self-renewal. Additionally, cells cultured in PNJ scaffolds exhibited increased expression of the transcription factor HIF2α, which was not observed in neurosphere culture, providing a potential mechanistic basis for differences in radio-resistance. Differences in PNJ regulation of HIF2α in irradiated and untreated conditions also offered evidence of stem plasticity. These data show PNJ scaffolds provide a unique biomaterial for evaluating dynamic microenvironmental regulation of GSC self-renewal, radioresistance, and stem plasticity."],"journal":["Biomaterials science"],"pubmed_title":["PNJ scaffolds promote microenvironmental regulation of glioblastoma stem-like cell enrichment and radioresistance."],"pmcid":["PMC8939461"],"funding_grant_id":["R01 NS111292","R01 NS088648","R01HD099543","R01NS111292","R01NS088648","R01 HD099543"],"pubmed_authors":["McNamara JB","Heffernan JM","Mehta S","Sirianni RW","Vernon BL"],"additional_accession":[]},"is_claimable":false,"name":"PNJ scaffolds promote microenvironmental regulation of glioblastoma stem-like cell enrichment and radioresistance.","description":"Glioblastoma (GBM) brain tumors contain a subpopulation of self-renewing multipotent Glioblastoma stem-like cells (GSCs) that are believed to drive the near inevitable recurrence of GBM. We previously engineered temperature responsive scaffolds based on the polymer poly(<i>N</i>-isopropylacrylamide-<i>co</i>-Jeffamine M-1000 acrylamide) (PNJ) for the purpose of enriching GSCs <i>in vitro</i> from patient-derived samples. Here, we used PNJ scaffolds to study microenvironmental regulation of self-renewal and radiation response in patient-derived GSCs representing classical and proneural subtypes. GSC self-renewal was regulated by the composition of PNJ scaffolds and varied with cell type. PNJ scaffolds protected against radiation-induced cell death, particularly in conditions that also promoted GSC self-renewal. Additionally, cells cultured in PNJ scaffolds exhibited increased expression of the transcription factor HIF2α, which was not observed in neurosphere culture, providing a potential mechanistic basis for differences in radio-resistance. Differences in PNJ regulation of HIF2α in irradiated and untreated conditions also offered evidence of stem plasticity. These data show PNJ scaffolds provide a unique biomaterial for evaluating dynamic microenvironmental regulation of GSC self-renewal, radioresistance, and stem plasticity.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2025-04-05T09:05:11.474Z","creation":"2025-04-05T09:05:11.474Z"},"accession":"S-EPMC8939461","cross_references":{"pubmed":["34994746"],"doi":["10.1039/d0bm01169j"]}}