{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["1205"],"submitter":["Lopez-Valls M"],"funding":["Instituto de Salud Carlos III","Ministerio de Economía y Competitividad"],"pubmed_abstract":["The COVID-19 pandemic has brought to light the need for fast and sensitive detection methods to prevent the spread of pathogens. The scientific community is making a great effort to design new molecular detection methods suitable for fast point-of-care applications. In this regard, a variety of approaches have been developed or optimized, including isothermal amplification of viral nucleic acids, CRISPR-mediated target recognition, and read-out systems based on nanomaterials. Herein, we present CASCADE (CRISPR/CAS-based Colorimetric nucleic Acid DEtection), a sensing system for fast and specific naked-eye detection of SARS-CoV-2 RNA. In this approach, viral RNA is recognized by the LwaCas13a CRISPR protein, which activates its collateral RNase activity. Upon target recognition, Cas13a clea"],"journal":["Analytica chimica acta"],"pagination":["339749"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8939626"],"repository":["biostudies-literature"],"pubmed_title":["CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticles."],"pmcid":["PMC8939626"],"pubmed_authors":["Castellanos M","Lopez-Valls M","Rodriguez-Diaz C","Canton R","Coloma R","Abreu M","Miranda R","Somoza A","Martinez-Garay C","Escalona-Noguero C","Milan-Rois P","Pardo D","Galan JC","Sot B"],"additional_accession":[]},"is_claimable":false,"name":"CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticles.","description":"The COVID-19 pandemic has brought to light the need for fast and sensitive detection methods to prevent the spread of pathogens. The scientific community is making a great effort to design new molecular detection methods suitable for fast point-of-care applications. In this regard, a variety of approaches have been developed or optimized, including isothermal amplification of viral nucleic acids, CRISPR-mediated target recognition, and read-out systems based on nanomaterials. Herein, we present CASCADE (CRISPR/CAS-based Colorimetric nucleic Acid DEtection), a sensing system for fast and specific naked-eye detection of SARS-CoV-2 RNA. In this approach, viral RNA is recognized by the LwaCas13a CRISPR protein, which activates its collateral RNase activity. Upon target recognition, Cas13a clea","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-26T04:40:34.889Z","creation":"2025-04-06T11:13:01.457Z"},"accession":"S-EPMC8939626","cross_references":{"pubmed":["35414398"],"doi":["10.1016/j.aca.2022.339749"]}}