biostudies-literatureZhang YUKRI | Biotechnology and Biological Sciences Research CouncilBiotechnology and Biological Sciences Research Councile0202721https://www.ebi.ac.uk/biostudies/studies/S-EPMC8941929biostudies-literatureUnknown96(6)Marek's disease virus (MDV) is a member of the genus Mardivirus in the subfamily <i>Alphaherpesvirinae</i>. There are three different serotypes of MDV designated as MDV-1 (<i>Gallid herpesvirus</i> type 2), MDV-2 (<i>Gallid herpesvirus</i> type 3), and MDV-3 (Meleagrid herpesvirus 1, herpesvirus of turkeys, HVT). MDV-1 is the only serotype that induces Marek's disease (MD), a lymphoproliferative disorder resulting in aggressive T-cell lymphomas and paralytic symptoms. In the lymphomas and lymphoblastoid cell lines (LCL) derived from them, MDV establishes latent infection with limited viral gene expression. The latent viral genome in LCL can be activated by co-cultivation with chicken embryo fibroblast (CEF) monolayers. MSB-1, one of the first MDV-transformed LCL established from the spleniJournal of virologyTargeted Deletion of Glycoprotein B Gene by CRISPR/Cas9 Nuclease Inhibits <i>Gallid herpesvirus</i> Type 3 in Dually Infected Marek's Disease Virus-Transformed Lymphoblastoid Cell Line MSB-1.PMC8941929BBS/OS/NW/000007BB/R007896/1BBS/E/I/00007039BBS/E/I/00007032BB/R007632/1Li WNair VYao YTang NZhang YMoffat KfalseTargeted Deletion of Glycoprotein B Gene by CRISPR/Cas9 Nuclease Inhibits <i>Gallid herpesvirus</i> Type 3 in Dually Infected Marek's Disease Virus-Transformed Lymphoblastoid Cell Line MSB-1.Marek's disease virus (MDV) is a member of the genus Mardivirus in the subfamily <i>Alphaherpesvirinae</i>. There are three different serotypes of MDV designated as MDV-1 (<i>Gallid herpesvirus</i> type 2), MDV-2 (<i>Gallid herpesvirus</i> type 3), and MDV-3 (Meleagrid herpesvirus 1, herpesvirus of turkeys, HVT). MDV-1 is the only serotype that induces Marek's disease (MD), a lymphoproliferative disorder resulting in aggressive T-cell lymphomas and paralytic symptoms. In the lymphomas and lymphoblastoid cell lines (LCL) derived from them, MDV establishes latent infection with limited viral gene expression. The latent viral genome in LCL can be activated by co-cultivation with chicken embryo fibroblast (CEF) monolayers. MSB-1, one of the first MDV-transformed LCL established from the spleni2022-01-01T00:00:00Z2022 Mar2025-04-04T07:55:08.05Z2025-04-04T07:55:08.05ZS-EPMC89419293510737710.1128/jvi.02027-21