{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xiong Q"],"funding":["Fundamental Research Funds for the Central Universities","National Natural Science Foundation of China","Sichuan University"],"pagination":["3571-3581"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8943893"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(12)"],"pubmed_abstract":["Genetic incorporation of novel noncanonical amino acids (ncAAs) that are specialized for the photo-click reaction allows the precisely orthogonal and site-specific functionalization of proteins in living cells under photo-control. However, the development of a r̲ing-strain <i>i̲n situ</i> l̲oadable d̲ipolarophile (RILD) as a genetically encodable reporter for photo-click bioconjugation with spatiotemporal controllability is quite rare. Herein, we report the design and synthesis of a photo-switchable d̲ib̲enzo[<i>b</i>,<i>f</i>][1,4,5]t̲hiad̲iazepine-based a̲lanine (DBTDA) ncAA, together with the directed evolution of a pyrrolysyl-tRNA synthetase/tRNA<sub>CUA</sub> pair (PylRS/tRNA<sub>CUA</sub>), to encode the DBTDA into recombinant proteins as a RILD in living <i>E. coli</i> cells. The fa"],"journal":["Chemical science"],"pubmed_title":["Expanding the functionality of proteins with genetically encoded dibenzo[<i>b</i>,<i>f</i>][1,4,5]thiadiazepine: a photo-transducer for photo-click decoration."],"pmcid":["PMC8943893"],"funding_grant_id":["2020SCUNL105","22077090","20826041D4117","22001181"],"pubmed_authors":["Wang C","Xiong Q","Fu J","Li B","Yu Z","Shen X","Zheng T","Zhao X"],"additional_accession":[]},"is_claimable":false,"name":"Expanding the functionality of proteins with genetically encoded dibenzo[<i>b</i>,<i>f</i>][1,4,5]thiadiazepine: a photo-transducer for photo-click decoration.","description":"Genetic incorporation of novel noncanonical amino acids (ncAAs) that are specialized for the photo-click reaction allows the precisely orthogonal and site-specific functionalization of proteins in living cells under photo-control. However, the development of a r̲ing-strain <i>i̲n situ</i> l̲oadable d̲ipolarophile (RILD) as a genetically encodable reporter for photo-click bioconjugation with spatiotemporal controllability is quite rare. Herein, we report the design and synthesis of a photo-switchable d̲ib̲enzo[<i>b</i>,<i>f</i>][1,4,5]t̲hiad̲iazepine-based a̲lanine (DBTDA) ncAA, together with the directed evolution of a pyrrolysyl-tRNA synthetase/tRNA<sub>CUA</sub> pair (PylRS/tRNA<sub>CUA</sub>), to encode the DBTDA into recombinant proteins as a RILD in living <i>E. coli</i> cells. The fa","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-05-09T15:10:36.12Z","creation":"2024-11-05T17:03:08.591Z"},"accession":"S-EPMC8943893","cross_references":{"pubmed":["35432856"],"doi":["10.1039/d1sc05710c"]}}