biostudies-literatureLiu YPublic Welfare Foundation of Ningbo CityLi Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Development FundNational Natural Science Foundation of ChinaZhejiang Basic Public Welfare Research ProjectHigher Education Discipline Innovation ProjectNingbo Key Science and Technology Development Program9909-9916https://www.ebi.ac.uk/biostudies/studies/S-EPMC8945076biostudies-literatureUnknown7(11)Two rare tetracyclic skeleton alkaloids named perinadines B and C (<b>1</b> and <b>2</b>) were isolated as mixtures of epimers from the marine-derived <i>Aspergillus</i> sp. LS116 driven by molecular networking. The planar structures of <b>1</b> and <b>2</b> were characterized by comprehensive spectroscopic data. Additionally, compounds <b>1</b> and <b>2</b> showed moderate <i>in vitro</i> antibacterial activity against <i>Bacillus subtilis</i> with minimum inhibitory concentration values of 32 and 64 μg/mL, respectively. Besides, both of the compounds were evaluated for anti-inflammatory activities in an <i>in vivo</i> zebra fish model.ACS omegaMolecular Networking-Driven Discovery of Antibacterial Perinadines, New Tetracyclic Alkaloids from the Marine Sponge-Derived Fungus <i>Aspergillus</i> sp.PMC89450762021Z046421761014170616742006104LGF22B020002D160132021S059Liu YHe SWu BYan XDing LShi YfalseMolecular Networking-Driven Discovery of Antibacterial Perinadines, New Tetracyclic Alkaloids from the Marine Sponge-Derived Fungus <i>Aspergillus</i> sp.Two rare tetracyclic skeleton alkaloids named perinadines B and C (<b>1</b> and <b>2</b>) were isolated as mixtures of epimers from the marine-derived <i>Aspergillus</i> sp. LS116 driven by molecular networking. The planar structures of <b>1</b> and <b>2</b> were characterized by comprehensive spectroscopic data. Additionally, compounds <b>1</b> and <b>2</b> showed moderate <i>in vitro</i> antibacterial activity against <i>Bacillus subtilis</i> with minimum inhibitory concentration values of 32 and 64 μg/mL, respectively. Besides, both of the compounds were evaluated for anti-inflammatory activities in an <i>in vivo</i> zebra fish model.2022-01-01T00:00:00Z2022 Mar2026-05-30T15:02:52.581Z2025-04-04T23:04:39.529ZS-EPMC89450763535030410.1021/acsomega.2c00402