{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Martin K"],"funding":["NIA NIH HHS","NIDDK NIH HHS","U.S. Department of Defense","National Cancer Institute","NCI NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS","Division of Graduate Education"],"pagination":["4452-4461"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8945255"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(9)"],"pubmed_abstract":["Recent advances in sample preparation enable label-free mass spectrometry (MS)-based proteome profiling of small numbers of mammalian cells. However, specific devices are often required to downscale sample processing volume from the standard 50-200 μL to sub-μL for effective nanoproteomics, which greatly impedes the implementation of current nanoproteomics methods by the proteomics research community. Herein, we report a facile one-pot nanoproteomics method termed SOPs-MS (surfactant-assisted one-pot sample processing at the standard volume coupled with MS) for convenient robust proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOPs-MS for label-free single-cell proteomics at a low μL volume, we have systematically evaluated "],"journal":["Journal of proteome research"],"pubmed_title":["Facile One-Pot Nanoproteomics for Label-Free Proteome Profiling of 50-1000 Mammalian Cells."],"pmcid":["PMC8945255"],"funding_grant_id":["U54CA217378","P41GM103493","W81XWH-16-1-0021","U01 DK121289","U19 AG065156","R21 CA223715","T32 CA009054","UG3CA256967","U01CA227544","U01 CA227544","P41 GM103493","U54 CA217378","R21CA223715","R01GM090056","UG3 CA256967","P30 CA062203","DGE-1321846","P41 GM103533","P30CA062203"],"pubmed_authors":["Qian WJ","Lin TT","Smith RD","Chrisler WB","Waterman ML","Sontag RL","Yang B","Shi T","Lu YJ","Rodland KD","Liu T","Orton DJ","Zhang T","Tsai CF","Martin K","Zhao R","Wiley HS","Habowski AN"],"additional_accession":[]},"is_claimable":false,"name":"Facile One-Pot Nanoproteomics for Label-Free Proteome Profiling of 50-1000 Mammalian Cells.","description":"Recent advances in sample preparation enable label-free mass spectrometry (MS)-based proteome profiling of small numbers of mammalian cells. However, specific devices are often required to downscale sample processing volume from the standard 50-200 μL to sub-μL for effective nanoproteomics, which greatly impedes the implementation of current nanoproteomics methods by the proteomics research community. Herein, we report a facile one-pot nanoproteomics method termed SOPs-MS (surfactant-assisted one-pot sample processing at the standard volume coupled with MS) for convenient robust proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOPs-MS for label-free single-cell proteomics at a low μL volume, we have systematically evaluated ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-04-04T07:45:29.895Z","creation":"2025-04-04T07:45:29.895Z"},"accession":"S-EPMC8945255","cross_references":{"pubmed":["34351778"],"doi":["10.1021/acs.jproteome.1c00403"]}}