biostudies-literatureMartin KNIA NIH HHSNIDDK NIH HHSU.S. Department of DefenseNational Cancer InstituteNCI NIH HHSNational Institute of General Medical SciencesNIGMS NIH HHSDivision of Graduate Education4452-4461https://www.ebi.ac.uk/biostudies/studies/S-EPMC8945255biostudies-literatureUnknown20(9)Recent advances in sample preparation enable label-free mass spectrometry (MS)-based proteome profiling of small numbers of mammalian cells. However, specific devices are often required to downscale sample processing volume from the standard 50-200 μL to sub-μL for effective nanoproteomics, which greatly impedes the implementation of current nanoproteomics methods by the proteomics research community. Herein, we report a facile one-pot nanoproteomics method termed SOPs-MS (surfactant-assisted one-pot sample processing at the standard volume coupled with MS) for convenient robust proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOPs-MS for label-free single-cell proteomics at a low μL volume, we have systematically evaluated Journal of proteome researchFacile One-Pot Nanoproteomics for Label-Free Proteome Profiling of 50-1000 Mammalian Cells.PMC8945255U54CA217378P41GM103493W81XWH-16-1-0021U01 DK121289U19 AG065156R21 CA223715T32 CA009054UG3CA256967U01CA227544U01 CA227544P41 GM103493U54 CA217378R21CA223715R01GM090056UG3 CA256967P30 CA062203DGE-1321846P41 GM103533P30CA062203Qian WJLin TTSmith RDChrisler WBWaterman MLSontag RLYang BShi TLu YJRodland KDLiu TOrton DJZhang TTsai CFMartin KZhao RWiley HSHabowski ANfalseFacile One-Pot Nanoproteomics for Label-Free Proteome Profiling of 50-1000 Mammalian Cells.Recent advances in sample preparation enable label-free mass spectrometry (MS)-based proteome profiling of small numbers of mammalian cells. However, specific devices are often required to downscale sample processing volume from the standard 50-200 μL to sub-μL for effective nanoproteomics, which greatly impedes the implementation of current nanoproteomics methods by the proteomics research community. Herein, we report a facile one-pot nanoproteomics method termed SOPs-MS (surfactant-assisted one-pot sample processing at the standard volume coupled with MS) for convenient robust proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOPs-MS for label-free single-cell proteomics at a low μL volume, we have systematically evaluated 2021-01-01T00:00:00Z2021 Sep2025-04-04T07:45:29.895Z2025-04-04T07:45:29.895ZS-EPMC89452553435177810.1021/acs.jproteome.1c00403