{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(18)"],"submitter":["Schmidt S"],"pubmed_abstract":["Morbidity and mortality of Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs) are mainly determined by thromboembolic complications. Thrombus formation is facilitated by a neutrophil-specific form of cell death linked to neutrophil extracellular trap (NET) formation (NETosis). Preclinical and clinical data suggested a potential link between NETosis and thrombosis in MPNs. In this study, we aimed to define the impact of NETosis on clinical end points in a large MPN cohort. NETosis was induced in vitro by ionomycin and quantified by enzyme-linked immunosorbent assay-based nucleosome release assays as well as fluorescent staining of free DNA in samples from 103 MPN patients and 28 healthy donors. NETosis rate was correlated with a broad set of clinical data, such as MPN subt"],"journal":["Blood advances"],"pagination":["3515-3527"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8945589"],"repository":["biostudies-literature"],"pubmed_title":["Increased levels of NETosis in myeloproliferative neoplasms are not linked to thrombotic events."],"pmcid":["PMC8945589"],"pubmed_authors":["Wolf D","Gunsilius E","Schmidt S","Daniliants D","Hiller E","Feistritzer C"],"additional_accession":[]},"is_claimable":false,"name":"Increased levels of NETosis in myeloproliferative neoplasms are not linked to thrombotic events.","description":"Morbidity and mortality of Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs) are mainly determined by thromboembolic complications. Thrombus formation is facilitated by a neutrophil-specific form of cell death linked to neutrophil extracellular trap (NET) formation (NETosis). Preclinical and clinical data suggested a potential link between NETosis and thrombosis in MPNs. In this study, we aimed to define the impact of NETosis on clinical end points in a large MPN cohort. NETosis was induced in vitro by ionomycin and quantified by enzyme-linked immunosorbent assay-based nucleosome release assays as well as fluorescent staining of free DNA in samples from 103 MPN patients and 28 healthy donors. NETosis rate was correlated with a broad set of clinical data, such as MPN subt","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-04-05T14:43:37.168Z","creation":"2025-04-05T14:43:37.168Z"},"accession":"S-EPMC8945589","cross_references":{"pubmed":["34464975"],"doi":["10.1182/bloodadvances.2020004061"]}}