biostudies-literatureUnknown5(18)Schmidt SMorbidity and mortality of Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs) are mainly determined by thromboembolic complications. Thrombus formation is facilitated by a neutrophil-specific form of cell death linked to neutrophil extracellular trap (NET) formation (NETosis). Preclinical and clinical data suggested a potential link between NETosis and thrombosis in MPNs. In this study, we aimed to define the impact of NETosis on clinical end points in a large MPN cohort. NETosis was induced in vitro by ionomycin and quantified by enzyme-linked immunosorbent assay-based nucleosome release assays as well as fluorescent staining of free DNA in samples from 103 MPN patients and 28 healthy donors. NETosis rate was correlated with a broad set of clinical data, such as MPN subtBlood advances3515-3527https://www.ebi.ac.uk/biostudies/studies/S-EPMC8945589biostudies-literatureIncreased levels of NETosis in myeloproliferative neoplasms are not linked to thrombotic events.PMC8945589Wolf DGunsilius ESchmidt SDaniliants DHiller EFeistritzer CfalseIncreased levels of NETosis in myeloproliferative neoplasms are not linked to thrombotic events.Morbidity and mortality of Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs) are mainly determined by thromboembolic complications. Thrombus formation is facilitated by a neutrophil-specific form of cell death linked to neutrophil extracellular trap (NET) formation (NETosis). Preclinical and clinical data suggested a potential link between NETosis and thrombosis in MPNs. In this study, we aimed to define the impact of NETosis on clinical end points in a large MPN cohort. NETosis was induced in vitro by ionomycin and quantified by enzyme-linked immunosorbent assay-based nucleosome release assays as well as fluorescent staining of free DNA in samples from 103 MPN patients and 28 healthy donors. NETosis rate was correlated with a broad set of clinical data, such as MPN subt2021-01-01T00:00:00Z2021 Sep2025-04-05T14:43:37.168Z2025-04-05T14:43:37.168ZS-EPMC89455893446497510.1182/bloodadvances.2020004061