biostudies-literatureUnknown2022Chang C<i>Background</i>/<i>Aim</i>. MircoRNA-4731-5p (miR-4731-5p) is a new miRNA involved in different human cancers, but its function has not been clarified in non-small-cell lung cancer (NSCLC). The present study attended to resolve the role of miR-4731-5p in NSCLC. <i>Materials and Methods</i>. The expression level of miR-4731-5p or ribosomal protein large P0 (RPLP0) and NSCLC clinicopathologic characteristics were analyzed. The binding between miR-4731-5p and RPLP0 was confirmed by TargetScan prediction and luciferase reporter experiment. Also, the probable role of miR-4731-5p in NSCLC via RPLP0 was elaborated by the MTT, western blotting, immunofluorescence, transwell, flow cytometry, and TUNEL assays. Moreover, <i>in vivo</i> verification was conducted in xenografted nude mice. <i>Results</i>. The level of miR-4731-5p was notably declined <i>in vivo</i> and <i>in vitro</i>, which was involved in the prognosis of lung cancer patients. The miR-4731-5p mimic could remarkably restrain cell viability, invasion, and the translational expression level of vimentin and e-cadherin, with promoted cell apoptosis in NSCLC, which were notably reversed by RPLP0 overexpression. <i>Conclusion</i>. miR-4731-5p/RPLP0 axis might be an underlying therapeutic target for NSCLC.Journal of oncology3793318https://www.ebi.ac.uk/biostudies/studies/S-EPMC8947863biostudies-literaturemiR-4731-5p Enhances Apoptosis and Alleviates Epithelial-Mesenchymal Transition through Targeting RPLP0 in Non-Small-Cell Lung Cancer.PMC8947863Xu MChang CfalsemiR-4731-5p Enhances Apoptosis and Alleviates Epithelial-Mesenchymal Transition through Targeting RPLP0 in Non-Small-Cell Lung Cancer.<i>Background</i>/<i>Aim</i>. MircoRNA-4731-5p (miR-4731-5p) is a new miRNA involved in different human cancers, but its function has not been clarified in non-small-cell lung cancer (NSCLC). The present study attended to resolve the role of miR-4731-5p in NSCLC. <i>Materials and Methods</i>. The expression level of miR-4731-5p or ribosomal protein large P0 (RPLP0) and NSCLC clinicopathologic characteristics were analyzed. The binding between miR-4731-5p and RPLP0 was confirmed by TargetScan prediction and luciferase reporter experiment. Also, the probable role of miR-4731-5p in NSCLC via RPLP0 was elaborated by the MTT, western blotting, immunofluorescence, transwell, flow cytometry, and TUNEL assays. Moreover, <i>in vivo</i> verification was conducted in xenografted nude mice. <i>Results</i>. The level of miR-4731-5p was notably declined <i>in vivo</i> and <i>in vitro</i>, which was involved in the prognosis of lung cancer patients. The miR-4731-5p mimic could remarkably restrain cell viability, invasion, and the translational expression level of vimentin and e-cadherin, with promoted cell apoptosis in NSCLC, which were notably reversed by RPLP0 overexpression. <i>Conclusion</i>. miR-4731-5p/RPLP0 axis might be an underlying therapeutic target for NSCLC.2022-01-01T00:00:00Z20222025-04-19T09:23:14.396Z2025-04-19T09:23:14.396ZS-EPMC89478633534239810.1155/2022/3793318