biostudies-literatureUnknown54(2)Elzaki SHumboldt-Universität zu BerlinThe improvement of milk production of indigenous Sudanese cattle such as Bos indicus Butana and its cross with Holstein is a major goal of the Sudanese government to ensure sufficient healthy nutrition in the country. In this study, we investigated the K232A polymorphism of diacylglycerol acyltransferase (DGAT1), a well-known modulator of milk production in other breeds. We determined allele frequencies and the allele effects on milk production. Therefore, 93 purebred Butana and 203 Butana × Holstein crossbred cattle were genotyped using competitive allele-specific PCR assays. Association analysis was performed using a linear mixed model in R. In purebred Butana cattle, the lysine DGAT1 protein variant K232, which is found to be associated with higher fat and protein contents, as well as hTropical animal health and production142https://www.ebi.ac.uk/biostudies/studies/S-EPMC8948139biostudies-literatureEffects of DGAT1 on milk performance in Sudanese Butana × Holstein crossbred cattle.PMC8948139Brockmann GAArends DElzaki SKorkuc PReissmann MfalseEffects of DGAT1 on milk performance in Sudanese Butana × Holstein crossbred cattle.The improvement of milk production of indigenous Sudanese cattle such as Bos indicus Butana and its cross with Holstein is a major goal of the Sudanese government to ensure sufficient healthy nutrition in the country. In this study, we investigated the K232A polymorphism of diacylglycerol acyltransferase (DGAT1), a well-known modulator of milk production in other breeds. We determined allele frequencies and the allele effects on milk production. Therefore, 93 purebred Butana and 203 Butana × Holstein crossbred cattle were genotyped using competitive allele-specific PCR assays. Association analysis was performed using a linear mixed model in R. In purebred Butana cattle, the lysine DGAT1 protein variant K232, which is found to be associated with higher fat and protein contents, as well as h2022-01-01T00:00:00Z2022 Mar2025-04-26T18:29:21.674Z2025-04-06T15:49:25.201ZS-EPMC89481393533236210.1007/s11250-022-03141-7