biostudies-literatureGotzhein FDeutsche ForschungsgemeinschaftDr. Werner Jackstädt-StiftungBurkhard Meyer Stiftung3160https://www.ebi.ac.uk/biostudies/studies/S-EPMC8948791biostudies-literatureUnknown23(6)Hematopoietic stem cell transplantation (HSCT) represents the only curative treatment option for numerous hematologic malignancies. While the influence of donor age and the composition of the graft have already been examined in clinical and preclinical studies, little information is available on the extent to which different hematological subpopulations contribute to the dynamics of the reconstitution process and on whether and how these contributions are altered with age. In a murine model of HSCT, we therefore simultaneously tracked different cultivated and transduced hematopoietic stem and progenitor cell (HSPC) populations using a multicolor-coded barcode system (BC32). We studied a series of age-matched and age-mismatched transplantations and compared the influence of age on the reconInternational journal of molecular sciencesThe Reconstitution Dynamics of Cultivated Hematopoietic Stem Cells and Progenitors Is Independent of Age.PMC8948791GL 721/1-1-CO 1692/1-1Fehse BGlauche IAranyossy TCornils KGotzhein FSonntag TThaden VThielecke LMuller IfalseThe Reconstitution Dynamics of Cultivated Hematopoietic Stem Cells and Progenitors Is Independent of Age.Hematopoietic stem cell transplantation (HSCT) represents the only curative treatment option for numerous hematologic malignancies. While the influence of donor age and the composition of the graft have already been examined in clinical and preclinical studies, little information is available on the extent to which different hematological subpopulations contribute to the dynamics of the reconstitution process and on whether and how these contributions are altered with age. In a murine model of HSCT, we therefore simultaneously tracked different cultivated and transduced hematopoietic stem and progenitor cell (HSPC) populations using a multicolor-coded barcode system (BC32). We studied a series of age-matched and age-mismatched transplantations and compared the influence of age on the recon2022-01-01T00:00:00Z2022 Mar2025-04-04T08:24:40.371Z2025-04-04T08:24:40.371ZS-EPMC89487913532857910.3390/ijms23063160