{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jiang Z"],"funding":["National Key R&amp;D Program of China; National Natural Science Foundation of China; Oceanic Interdisciplinary Program of Shanghai Jiao Tong University"],"pagination":["1758"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8948812"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(6)"],"pubmed_abstract":["Prime editor (PE), a versatile editor that allows the insertion and deletion of arbitrary sequences, and all 12-point mutations without double-strand breaks (DSB) and a donor template, dramatically enhances research capabilities. PE combines nickase Cas9(H840A) and reverse transcriptase (RT), along with prime editing guide RNA (pegRNA). It has been reported in several plant species, but a weak editing efficiency has led to a decrease in applications. This study reports an optimized-prime editor (O-PE) for endogenous gene editing in <i>Arabidopsis thaliana</i> cells, with an average 1.15% editing efficiency, which is 16.4-fold higher than previously reported. Meanwhile, we observed an increase in indels when testing alternative reverse transcriptase and found out that nCas9(H840A) fused to "],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Development and Optimization of CRISPR Prime Editing System in Photoautotrophic Cells."],"pmcid":["PMC8948812"],"funding_grant_id":["2018YFA0901200; 31870071; SL2020MS028"],"pubmed_authors":["Jiang Y","Jiang Z","Xiao Y","Liu R","Zhang S","Abdullah"],"additional_accession":[]},"is_claimable":false,"name":"Development and Optimization of CRISPR Prime Editing System in Photoautotrophic Cells.","description":"Prime editor (PE), a versatile editor that allows the insertion and deletion of arbitrary sequences, and all 12-point mutations without double-strand breaks (DSB) and a donor template, dramatically enhances research capabilities. PE combines nickase Cas9(H840A) and reverse transcriptase (RT), along with prime editing guide RNA (pegRNA). It has been reported in several plant species, but a weak editing efficiency has led to a decrease in applications. This study reports an optimized-prime editor (O-PE) for endogenous gene editing in <i>Arabidopsis thaliana</i> cells, with an average 1.15% editing efficiency, which is 16.4-fold higher than previously reported. Meanwhile, we observed an increase in indels when testing alternative reverse transcriptase and found out that nCas9(H840A) fused to ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-26T10:15:35.037Z","creation":"2025-04-06T13:16:38.232Z"},"accession":"S-EPMC8948812","cross_references":{"pubmed":["35335122"],"doi":["10.3390/molecules27061758"]}}