biostudies-literatureWu XGuangdong Basic and Applied Basic Research FoundationKey Research and Development Program of Guangdong Provincethe Chief expert Project of Agricultural Industry Technology system in Guangdong Provincethe National Natural Science Foundation of China591https://www.ebi.ac.uk/biostudies/studies/S-EPMC8948910biostudies-literatureUnknown14(3)Porcine epidemic diarrhea virus (PEDV) is a coronavirus currently widespread worldwide in the swine industry. Since PEDV was discovered in China in 1984, it has caused huge economic losses in the swine industry. PEDV can infect pigs of all ages, but piglets have the highest infection with a death rate as high as 100%, and the clinical symptoms are watery diarrhea, vomiting, and dehydration. At present, there is not any report on PEDV detection by RT-RAA. In this study, we developed an isothermal amplification technology by using reverse-transcription recombinase-aided amplification assay (RT-RAA) combined with portable instruments to achieve a molecular diagnosis of PEDV in clinical samples from China. By designing a pair of RT-RAA primers and probes based on the PEDV N gene, this method bVirusesDevelopment and Application of a Reverse-Transcription Recombinase-Aided Amplification Assay for Porcine Epidemic Diarrhea Virus.PMC894891031902252, 319726592019A15150120062019KJ1282020B020222001Liu YYan ZZhao QGao LZhang XChen FXie QWu XfalseDevelopment and Application of a Reverse-Transcription Recombinase-Aided Amplification Assay for Porcine Epidemic Diarrhea Virus.Porcine epidemic diarrhea virus (PEDV) is a coronavirus currently widespread worldwide in the swine industry. Since PEDV was discovered in China in 1984, it has caused huge economic losses in the swine industry. PEDV can infect pigs of all ages, but piglets have the highest infection with a death rate as high as 100%, and the clinical symptoms are watery diarrhea, vomiting, and dehydration. At present, there is not any report on PEDV detection by RT-RAA. In this study, we developed an isothermal amplification technology by using reverse-transcription recombinase-aided amplification assay (RT-RAA) combined with portable instruments to achieve a molecular diagnosis of PEDV in clinical samples from China. By designing a pair of RT-RAA primers and probes based on the PEDV N gene, this method b2022-01-01T00:00:00Z2022 Mar2025-04-22T09:47:58.966Z2025-04-05T23:17:01.569ZS-EPMC89489103533699810.3390/v14030591