{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sobotka P"],"funding":["Warsaw University of Technology"],"pagination":["2302"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8949262"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(6)"],"pubmed_abstract":["The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has completely disrupted people's lives. All over the world, many restrictions and precautions have been introduced to reduce the spread of coronavirus disease 2019 (COVID-19). Ultraviolet C (UV-C) radiation is widely used to disinfect rooms, surfaces, and medical tools; however, this paper presents novel results obtained for modern UV-C light-emitting diodes (LEDs), examining their effect on inhibiting the multiplication of viruses. The main goal of the work was to investigate how to most effectively use UV-C LEDs to inactivate viruses. We showed that UV-C radiation operating at a 275 nm wavelength is optimal for germicidal effectiveness in a time exposure (25-48 s) study: >3 log-reduction with the Kärber method and >6 log-reduction with UV spectrophotometry were noted. We used real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) to reliably estimate virus infectivity reduction after 275 nm UV-C disinfection. The relative quantification (RQ) of infectious particles detected after 40-48 s distinctly decreased. The irradiated viral RNAs were underexpressed compared to the untreated control virial amplicon (estimated as RQ = 1). In conclusion, this work provides the first experimental data on 275 nm UV-C in the inactivation of human coronavirus OC43 (HoV-OC43), showing the most potent germicidal effect without hazardous effect."],"journal":["Materials (Basel, Switzerland)"],"pubmed_title":["Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on Human Beta-Coronaviruses' Inactivation."],"pmcid":["PMC8949262"],"funding_grant_id":["504/04496/1050/45.010002"],"pubmed_authors":["Sobotka P","Staniszewska M","Uscilo K","Przychodzki M","Wolinski TR"],"additional_accession":[]},"is_claimable":false,"name":"Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on Human Beta-Coronaviruses' Inactivation.","description":"The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has completely disrupted people's lives. All over the world, many restrictions and precautions have been introduced to reduce the spread of coronavirus disease 2019 (COVID-19). Ultraviolet C (UV-C) radiation is widely used to disinfect rooms, surfaces, and medical tools; however, this paper presents novel results obtained for modern UV-C light-emitting diodes (LEDs), examining their effect on inhibiting the multiplication of viruses. The main goal of the work was to investigate how to most effectively use UV-C LEDs to inactivate viruses. We showed that UV-C radiation operating at a 275 nm wavelength is optimal for germicidal effectiveness in a time exposure (25-48 s) study: >3 log-reduction with the Kärber method and >6 log-reduction with UV spectrophotometry were noted. We used real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) to reliably estimate virus infectivity reduction after 275 nm UV-C disinfection. The relative quantification (RQ) of infectious particles detected after 40-48 s distinctly decreased. The irradiated viral RNAs were underexpressed compared to the untreated control virial amplicon (estimated as RQ = 1). In conclusion, this work provides the first experimental data on 275 nm UV-C in the inactivation of human coronavirus OC43 (HoV-OC43), showing the most potent germicidal effect without hazardous effect.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-19T00:52:19.015Z","creation":"2025-04-07T11:49:05.634Z"},"accession":"S-EPMC8949262","cross_references":{"pubmed":["35329754"],"doi":["10.3390/ma15062302"]}}