biostudies-literatureYu FMNatural Science Foundation of Guizhou ProvinceThe Second Tibetan Plateau Scientific Expedition and Research (STEP) Program299https://www.ebi.ac.uk/biostudies/studies/S-EPMC8950353biostudies-literatureUnknown8(3)True morels (Morchella, Pezizales) cultivated in soil are subject to complex influences from soil microbial communities. To explore the characteristics of soil microbial communities on morel cultivation, and evaluate whether these microbes are related to morel production, we collected 23 soil samples from four counties in Sichuan and Yunnan Provinces, China. Based on ITS and 16S rDNA amplicon sequencing, the alpha diversity analysis indicated that the biodiversity of morel cultivation soil showed a downward trend compared with the bare soil. The results also showed that there were no significant differences in soil microbial communities between OC (bare soil) and OO (after one-year suspension of sowing). This means that, after about one year of stopping sowing, the component and structure Journal of fungi (Basel, Switzerland)Morel Production Associated with Soil Nitrogen-Fixing and Nitrifying Microorganisms.PMC8950353292019312511043KFJ-STS-QYZD-171DBG62800092019QZKK0503Qian Ke Zhong Yin Di [2021]4031, Qian Ke He Zhi Cheng [2021] Generally 2002019HJ2096001006Yu FMLv MLZhu XTZhao QJayawardena RSThongklang NfalseMorel Production Associated with Soil Nitrogen-Fixing and Nitrifying Microorganisms.True morels (Morchella, Pezizales) cultivated in soil are subject to complex influences from soil microbial communities. To explore the characteristics of soil microbial communities on morel cultivation, and evaluate whether these microbes are related to morel production, we collected 23 soil samples from four counties in Sichuan and Yunnan Provinces, China. Based on ITS and 16S rDNA amplicon sequencing, the alpha diversity analysis indicated that the biodiversity of morel cultivation soil showed a downward trend compared with the bare soil. The results also showed that there were no significant differences in soil microbial communities between OC (bare soil) and OO (after one-year suspension of sowing). This means that, after about one year of stopping sowing, the component and structure 2022-01-01T00:00:00Z2022 Mar2025-04-26T13:10:51.126Z2025-04-06T14:08:08.242ZS-EPMC89503533533030010.3390/jof8030299