{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pujic P"],"funding":["Agence Nationale de la Recherche"],"pagination":["651"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8951365"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(3)"],"pubmed_abstract":["Omics are the most promising approaches to investigate microbes for which no genetic tools exist such as the nitrogen-fixing symbiotic <i>Frankia.</i> A proteogenomic analysis of symbiotic <i>Frankia alni</i> was done by comparing those proteins more and less abundant in <i>Alnus glutinosa</i> nodules relative to N<sub>2</sub>-fixing pure cultures with propionate as the carbon source. There were 250 proteins that were significantly overabundant in nodules at a fold change (FC) ≥ 2 threshold, and 1429 with the same characteristics in in vitro nitrogen-fixing pure culture. Nitrogenase, SuF (Fe-Su biogenesis) and hopanoid lipids synthesis determinants were the most overabundant proteins in symbiosis. Nitrogenase was found to constitute 3% of all <i>Frankia</i> proteins in nodules. Sod (supero"],"journal":["Microorganisms"],"pubmed_title":["The Proteogenome of Symbiotic <i>Frankia alni</i> in <i>Alnus glutinosa</i> Nodules."],"pmcid":["PMC8951365"],"funding_grant_id":["BugsInACell ANR-13-BSV7-0013-03"],"pubmed_authors":["Abrouk D","Fournier P","Alloisio N","Armengaud J","Normand P","Miotello G","Pujic P"],"additional_accession":[]},"is_claimable":false,"name":"The Proteogenome of Symbiotic <i>Frankia alni</i> in <i>Alnus glutinosa</i> Nodules.","description":"Omics are the most promising approaches to investigate microbes for which no genetic tools exist such as the nitrogen-fixing symbiotic <i>Frankia.</i> A proteogenomic analysis of symbiotic <i>Frankia alni</i> was done by comparing those proteins more and less abundant in <i>Alnus glutinosa</i> nodules relative to N<sub>2</sub>-fixing pure cultures with propionate as the carbon source. There were 250 proteins that were significantly overabundant in nodules at a fold change (FC) ≥ 2 threshold, and 1429 with the same characteristics in in vitro nitrogen-fixing pure culture. Nitrogenase, SuF (Fe-Su biogenesis) and hopanoid lipids synthesis determinants were the most overabundant proteins in symbiosis. Nitrogenase was found to constitute 3% of all <i>Frankia</i> proteins in nodules. Sod (supero","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-04-08T09:39:23.416Z","creation":"2025-04-19T22:35:58.49Z"},"accession":"S-EPMC8951365","cross_references":{"pubmed":["35336227"],"doi":["10.3390/microorganisms10030651"]}}