{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang Y"],"funding":["the Key National Project","Pear Modern Agro-industry Technology Research System"],"pagination":["576"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8952854"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(3)"],"pubmed_abstract":["Apple rubbery wood virus 2 (ARWV-2) and citrus virus A (CiVA) belong to a recently approved family <i>Phenuiviridae</i> in the order <i>Bunyavirales</i> and possess negative-sense single-stranded RNA genomes. In this study, the genome sequence of three ARWV-2 isolates (S17E2, LYC2, and LYXS) and a CiVA isolate (CiVA-P) infecting pear trees grown in China were characterized using high-throughput sequencing combined with conventional reverse-transcription PCR (RT-PCR) assays. The genome-wide nt sequence identities were above 93.6% among the ARWV-2 isolates and above 93% among CiVA isolates. Sequence comparisons showed that sequence diversity occurred in the 5' untranslated region of the ARWV-2 genome and the intergenic region of the CiVA genome. For the first time, this study revealed that A"],"journal":["Viruses"],"pubmed_title":["Molecular Characteristics and Incidence of Apple Rubbery Wood Virus 2 and Citrus Virus A Infecting Pear Trees in China."],"pmcid":["PMC8952854"],"funding_grant_id":["CARS-29-10","2019YFD1001800"],"pubmed_authors":["Yang Z","Hong N","Guo J","Zhang Z","Lv Y","Wang G","Li Q","Wang Y","Li L"],"additional_accession":[]},"is_claimable":false,"name":"Molecular Characteristics and Incidence of Apple Rubbery Wood Virus 2 and Citrus Virus A Infecting Pear Trees in China.","description":"Apple rubbery wood virus 2 (ARWV-2) and citrus virus A (CiVA) belong to a recently approved family <i>Phenuiviridae</i> in the order <i>Bunyavirales</i> and possess negative-sense single-stranded RNA genomes. In this study, the genome sequence of three ARWV-2 isolates (S17E2, LYC2, and LYXS) and a CiVA isolate (CiVA-P) infecting pear trees grown in China were characterized using high-throughput sequencing combined with conventional reverse-transcription PCR (RT-PCR) assays. The genome-wide nt sequence identities were above 93.6% among the ARWV-2 isolates and above 93% among CiVA isolates. Sequence comparisons showed that sequence diversity occurred in the 5' untranslated region of the ARWV-2 genome and the intergenic region of the CiVA genome. For the first time, this study revealed that A","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-04-08T17:32:17.302Z","creation":"2025-04-04T22:08:54.837Z"},"accession":"S-EPMC8952854","cross_references":{"pubmed":["35336983"],"doi":["10.3390/v14030576"]}}