biostudies-literatureWang Ythe Key National ProjectPear Modern Agro-industry Technology Research System576https://www.ebi.ac.uk/biostudies/studies/S-EPMC8952854biostudies-literatureUnknown14(3)Apple rubbery wood virus 2 (ARWV-2) and citrus virus A (CiVA) belong to a recently approved family <i>Phenuiviridae</i> in the order <i>Bunyavirales</i> and possess negative-sense single-stranded RNA genomes. In this study, the genome sequence of three ARWV-2 isolates (S17E2, LYC2, and LYXS) and a CiVA isolate (CiVA-P) infecting pear trees grown in China were characterized using high-throughput sequencing combined with conventional reverse-transcription PCR (RT-PCR) assays. The genome-wide nt sequence identities were above 93.6% among the ARWV-2 isolates and above 93% among CiVA isolates. Sequence comparisons showed that sequence diversity occurred in the 5' untranslated region of the ARWV-2 genome and the intergenic region of the CiVA genome. For the first time, this study revealed that AVirusesMolecular Characteristics and Incidence of Apple Rubbery Wood Virus 2 and Citrus Virus A Infecting Pear Trees in China.PMC8952854CARS-29-102019YFD1001800Yang ZHong NGuo JZhang ZLv YWang GLi QWang YLi LfalseMolecular Characteristics and Incidence of Apple Rubbery Wood Virus 2 and Citrus Virus A Infecting Pear Trees in China.Apple rubbery wood virus 2 (ARWV-2) and citrus virus A (CiVA) belong to a recently approved family <i>Phenuiviridae</i> in the order <i>Bunyavirales</i> and possess negative-sense single-stranded RNA genomes. In this study, the genome sequence of three ARWV-2 isolates (S17E2, LYC2, and LYXS) and a CiVA isolate (CiVA-P) infecting pear trees grown in China were characterized using high-throughput sequencing combined with conventional reverse-transcription PCR (RT-PCR) assays. The genome-wide nt sequence identities were above 93.6% among the ARWV-2 isolates and above 93% among CiVA isolates. Sequence comparisons showed that sequence diversity occurred in the 5' untranslated region of the ARWV-2 genome and the intergenic region of the CiVA genome. For the first time, this study revealed that A2022-01-01T00:00:00Z2022 Mar2026-04-08T17:32:17.302Z2025-04-04T22:08:54.837ZS-EPMC89528543533698310.3390/v14030576