{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kondratieva E"],"funding":["Russian Foundation for Basic Research"],"pagination":["2961"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8954342"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(6)"],"pubmed_abstract":["The role of neutrophils in tuberculosis infection remains less well studied compared to that of the CD4<sup>+</sup> T-lymphocytes and macrophages. Thus, alterations in <i>Mycobacterium tuberculosis</i> transcription profile following phagocytosis by neutrophils and how these shifts differ from those caused by macrophage phagocytosis remain unknown. We developed a mouse model that allows obtaining large amounts of either neutrophils or macrophages infected in vivo with <i>M. tuberculosis</i> for mycobacteria isolation in quantities sufficient for the whole genome RNA sequencing and aerosol challenge of mice. Here, we present: (i) the differences in transcription profiles of mycobacteria isolated from liquid cultures, neutrophils and macrophages infected in vivo; (ii) phenotypes of infection"],"journal":["International journal of molecular sciences"],"pubmed_title":["An In Vivo Model of Separate <i>M. tuberculosis</i> Phagocytosis by Neutrophils and Macrophages: Gene Expression Profiles in the Parasite and Disease Development in the Mouse Host."],"pmcid":["PMC8954342"],"funding_grant_id":["19-04-00058"],"pubmed_authors":["Rubakova E","Azhikina T","Kondratieva T","Kondratieva E","Majorov K","Skvortsova Y","Linge I","Grigorov A","Apt A"],"additional_accession":[]},"is_claimable":false,"name":"An In Vivo Model of Separate <i>M. tuberculosis</i> Phagocytosis by Neutrophils and Macrophages: Gene Expression Profiles in the Parasite and Disease Development in the Mouse Host.","description":"The role of neutrophils in tuberculosis infection remains less well studied compared to that of the CD4<sup>+</sup> T-lymphocytes and macrophages. Thus, alterations in <i>Mycobacterium tuberculosis</i> transcription profile following phagocytosis by neutrophils and how these shifts differ from those caused by macrophage phagocytosis remain unknown. We developed a mouse model that allows obtaining large amounts of either neutrophils or macrophages infected in vivo with <i>M. tuberculosis</i> for mycobacteria isolation in quantities sufficient for the whole genome RNA sequencing and aerosol challenge of mice. Here, we present: (i) the differences in transcription profiles of mycobacteria isolated from liquid cultures, neutrophils and macrophages infected in vivo; (ii) phenotypes of infection","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-04-08T17:32:36.621Z","creation":"2024-11-09T11:55:21.982Z"},"accession":"S-EPMC8954342","cross_references":{"pubmed":["35328388"],"doi":["10.3390/ijms23062961"]}}