biostudies-literatureKondratieva ERussian Foundation for Basic Research2961https://www.ebi.ac.uk/biostudies/studies/S-EPMC8954342biostudies-literatureUnknown23(6)The role of neutrophils in tuberculosis infection remains less well studied compared to that of the CD4⁺ T-lymphocytes and macrophages. Thus, alterations in <i>Mycobacterium tuberculosis</i> transcription profile following phagocytosis by neutrophils and how these shifts differ from those caused by macrophage phagocytosis remain unknown. We developed a mouse model that allows obtaining large amounts of either neutrophils or macrophages infected in vivo with <i>M. tuberculosis</i> for mycobacteria isolation in quantities sufficient for the whole genome RNA sequencing and aerosol challenge of mice. Here, we present: (i) the differences in transcription profiles of mycobacteria isolated from liquid cultures, neutrophils and macrophages infected in vivo; (ii) phenotypes of infectionInternational journal of molecular sciencesAn In Vivo Model of Separate <i>M. tuberculosis</i> Phagocytosis by Neutrophils and Macrophages: Gene Expression Profiles in the Parasite and Disease Development in the Mouse Host.PMC895434219-04-00058Rubakova EAzhikina TKondratieva TKondratieva EMajorov KSkvortsova YLinge IGrigorov AApt AfalseAn In Vivo Model of Separate <i>M. tuberculosis</i> Phagocytosis by Neutrophils and Macrophages: Gene Expression Profiles in the Parasite and Disease Development in the Mouse Host.The role of neutrophils in tuberculosis infection remains less well studied compared to that of the CD4⁺ T-lymphocytes and macrophages. Thus, alterations in <i>Mycobacterium tuberculosis</i> transcription profile following phagocytosis by neutrophils and how these shifts differ from those caused by macrophage phagocytosis remain unknown. We developed a mouse model that allows obtaining large amounts of either neutrophils or macrophages infected in vivo with <i>M. tuberculosis</i> for mycobacteria isolation in quantities sufficient for the whole genome RNA sequencing and aerosol challenge of mice. Here, we present: (i) the differences in transcription profiles of mycobacteria isolated from liquid cultures, neutrophils and macrophages infected in vivo; (ii) phenotypes of infection2022-01-01T00:00:00Z2022 Mar2026-04-08T17:32:36.621Z2024-11-09T11:55:21.982ZS-EPMC89543423532838810.3390/ijms23062961