biostudies-literatureLee HMMinistry of Agriculture, Food and Rural Affairs542https://www.ebi.ac.uk/biostudies/studies/S-EPMC8955018biostudies-literatureUnknown13(3)Gray mold disease caused by <i>Botrytis</i> in onions (<i>Allium cepa</i> L.) during growth and storage negatively affects their yield and quality. Exploring the genes related to gray mold resistance in onion and their application to the breeding of resistant onion lines will support effective and ecological control methods of the disease. Here, the genetic relationship of 54 onion lines based on random amplified polymorphic DNA (RAPD) and in vitro-cultured onion lines infected with gray mold were used for screening resistance and susceptibility traits. Two genetically related onion lines were selected, one with a resistant and one with a susceptible phenotype. In vitro gray mold infection was repeated with these two lines, and leaf samples were collected for gene expression studies in timGenesUsing Transcriptome Analysis to Explore Gray Mold Resistance-Related Genes in Onion (<i>Allium</i><i>cepa</i> L.).PMC8955018213007-05-5-SBB20Kim SGPark YDLee HMKim SJPark JSfalseUsing Transcriptome Analysis to Explore Gray Mold Resistance-Related Genes in Onion (<i>Allium</i><i>cepa</i> L.).Gray mold disease caused by <i>Botrytis</i> in onions (<i>Allium cepa</i> L.) during growth and storage negatively affects their yield and quality. Exploring the genes related to gray mold resistance in onion and their application to the breeding of resistant onion lines will support effective and ecological control methods of the disease. Here, the genetic relationship of 54 onion lines based on random amplified polymorphic DNA (RAPD) and in vitro-cultured onion lines infected with gray mold were used for screening resistance and susceptibility traits. Two genetically related onion lines were selected, one with a resistant and one with a susceptible phenotype. In vitro gray mold infection was repeated with these two lines, and leaf samples were collected for gene expression studies in tim2022-01-01T00:00:00Z2022 Mar2026-04-08T18:06:07.612Z2024-11-19T21:22:52.571ZS-EPMC89550183532809510.3390/genes13030542