{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Grzanka M"],"funding":["The National Science Center, Poland"],"pagination":["3262"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8955189"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(6)"],"pubmed_abstract":["Extracellular vesicles (EVs) are small, membranous structures involved in intercellular communication. Here, we analyzed the effects of thyroid cancer-derived EVs on the properties of normal thyroid cells and cells contributing to the tumor microenvironment. EVs isolated from thyroid cancer cell lines (CGTH, FTC-133, 8505c, TPC-1 and BcPAP) were used for treatment of normal thyroid cells (NTHY), as well as monocytes and endothelial cells (HUVEC). EVs' size/number were analyzed by flow cytometry and confocal microscopy. Gene expression, protein level and localization were investigated by qRT-PCR, WB and ICC/IF, respectively. Proliferation, migration and tube formation were analyzed. When compared with NTHY, CGTH and BcPAP secreted significantly more EVs. Treatment of NTHY with cancer-derive"],"journal":["International journal of molecular sciences"],"pubmed_title":["Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors?"],"pmcid":["PMC8955189"],"funding_grant_id":["2016/21/B/NZ5/00063"],"pubmed_authors":["Grzanka M","Gajda E","Czarnocka B","Stachurska-Skrodzka A","Adamiok-Ostrowska A"],"additional_accession":[]},"is_claimable":false,"name":"Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors?","description":"Extracellular vesicles (EVs) are small, membranous structures involved in intercellular communication. Here, we analyzed the effects of thyroid cancer-derived EVs on the properties of normal thyroid cells and cells contributing to the tumor microenvironment. EVs isolated from thyroid cancer cell lines (CGTH, FTC-133, 8505c, TPC-1 and BcPAP) were used for treatment of normal thyroid cells (NTHY), as well as monocytes and endothelial cells (HUVEC). EVs' size/number were analyzed by flow cytometry and confocal microscopy. Gene expression, protein level and localization were investigated by qRT-PCR, WB and ICC/IF, respectively. Proliferation, migration and tube formation were analyzed. When compared with NTHY, CGTH and BcPAP secreted significantly more EVs. Treatment of NTHY with cancer-derive","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-04T07:45:22.823Z","creation":"2025-04-04T07:45:22.823Z"},"accession":"S-EPMC8955189","cross_references":{"pubmed":["35328683"],"doi":["10.3390/ijms23063262"]}}