biostudies-literatureGrzanka MThe National Science Center, Poland3262https://www.ebi.ac.uk/biostudies/studies/S-EPMC8955189biostudies-literatureUnknown23(6)Extracellular vesicles (EVs) are small, membranous structures involved in intercellular communication. Here, we analyzed the effects of thyroid cancer-derived EVs on the properties of normal thyroid cells and cells contributing to the tumor microenvironment. EVs isolated from thyroid cancer cell lines (CGTH, FTC-133, 8505c, TPC-1 and BcPAP) were used for treatment of normal thyroid cells (NTHY), as well as monocytes and endothelial cells (HUVEC). EVs' size/number were analyzed by flow cytometry and confocal microscopy. Gene expression, protein level and localization were investigated by qRT-PCR, WB and ICC/IF, respectively. Proliferation, migration and tube formation were analyzed. When compared with NTHY, CGTH and BcPAP secreted significantly more EVs. Treatment of NTHY with cancer-deriveInternational journal of molecular sciencesExtracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors?PMC89551892016/21/B/NZ5/00063Grzanka MGajda ECzarnocka BStachurska-Skrodzka AAdamiok-Ostrowska AfalseExtracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors?Extracellular vesicles (EVs) are small, membranous structures involved in intercellular communication. Here, we analyzed the effects of thyroid cancer-derived EVs on the properties of normal thyroid cells and cells contributing to the tumor microenvironment. EVs isolated from thyroid cancer cell lines (CGTH, FTC-133, 8505c, TPC-1 and BcPAP) were used for treatment of normal thyroid cells (NTHY), as well as monocytes and endothelial cells (HUVEC). EVs' size/number were analyzed by flow cytometry and confocal microscopy. Gene expression, protein level and localization were investigated by qRT-PCR, WB and ICC/IF, respectively. Proliferation, migration and tube formation were analyzed. When compared with NTHY, CGTH and BcPAP secreted significantly more EVs. Treatment of NTHY with cancer-derive2022-01-01T00:00:00Z2022 Mar2025-04-04T07:45:22.823Z2025-04-04T07:45:22.823ZS-EPMC89551893532868310.3390/ijms23063262