biostudies-literatureLi QRCultivating Project for Young Scholar at Hubei University of MedicineInnovative Research Program for Graduates of Hubei University of MedicineNational Training Program of Innovation and Entrepreneurship for UndergraduatesNational Natural Science Foundation of ChinaHubei Provincial Natural Science FoundationHubei Province health and family planning scientific research project937-949https://www.ebi.ac.uk/biostudies/studies/S-EPMC8956315biostudies-literatureUnknown29(1)The present work aims to prove the concept of tumor-targeted drug delivery mediated by platelets. Doxorubicin (DOX) attached to nanodiamonds (ND-DOX) was investigated as the model payload drug of platelets. In vitro experiments first showed that ND-DOX could be loaded in mouse platelets in a dose-dependent manner with a markedly higher efficiency and capacity than free DOX. ND-DOX-loaded platelets (Plt@ND-DOX) maintained viability and ND-DOX could be stably held in the platelets for at least 4 hr. Next, mouse Lewis lung cancer cells were found to activate Plt@ND-DOX and thereby stimulate cargo unloading of Plt@ND-DOX. The unloaded ND-DOX was taken up by co-cultured cancer cells which consequently exhibited loss of viability, proliferation suppression and apoptosis. In vivo, Plt@ND-DOX dispDrug deliveryPlatelets are highly efficient and efficacious carriers for tumor-targeted nano-drug delivery.PMC89563152019QDJZR02202110929010WJ2019Z0102020QDJZR0022020CFB152YC202101681671818202113249001Li LGLi JWang CHan NXiao RCLi TFLiu YTang JMZhao LPeng XCWang MFXu HZKomatsu NLiu BLi QRChen XYu TTXu YHfalsePlatelets are highly efficient and efficacious carriers for tumor-targeted nano-drug delivery.The present work aims to prove the concept of tumor-targeted drug delivery mediated by platelets. Doxorubicin (DOX) attached to nanodiamonds (ND-DOX) was investigated as the model payload drug of platelets. In vitro experiments first showed that ND-DOX could be loaded in mouse platelets in a dose-dependent manner with a markedly higher efficiency and capacity than free DOX. ND-DOX-loaded platelets (Plt@ND-DOX) maintained viability and ND-DOX could be stably held in the platelets for at least 4 hr. Next, mouse Lewis lung cancer cells were found to activate Plt@ND-DOX and thereby stimulate cargo unloading of Plt@ND-DOX. The unloaded ND-DOX was taken up by co-cultured cancer cells which consequently exhibited loss of viability, proliferation suppression and apoptosis. In vivo, Plt@ND-DOX disp2022-01-01T00:00:00Z2022 Dec2025-04-04T09:10:00.99Z2025-04-04T09:10:00.99ZS-EPMC89563153531932110.1080/10717544.2022.2053762