biostudies-literatureRawlings SAIntramural NIH HHSNIDA NIH HHSNIAID NIH HHSNIMH NIH HHS621-630https://www.ebi.ac.uk/biostudies/studies/S-EPMC8957508biostudies-literatureUnknown36(5)<h4>Objective</h4>HIV infection disrupts the cytokine network and this disruption is not completely reversed by antiretroviral therapy (ART). Characterization of cytokine changes in blood and genital secretions is important for understanding HIV pathogenesis and the mechanisms of HIV sexual transmission. Here, we characterized the cytokine network in individuals longitudinally sampled before they began ART and after achieving suppression of HIV RNA.<h4>Methods</h4>We measured concentrations of 34 cytokine/chemokines using multiplex bead-based assay in blood and seminal plasma of 19 men with HIV-1 prior to and after viral suppression. We used Partial Least Squares Discriminant Analysis (PLS-DA) to visualize the difference in cytokine pattern between the time points. Any cytokines with VIP sAIDS (London, England)Effect of HIV suppression on the cytokine network in blood and seminal plasma.PMC8957508P30 MH062512T32 AI007384R01 AI147821Z99 HD999999DP2 DA051915P30 AI036214Fitzgerald WGianella SVanpouille CMargolis LWells ALisco ATorres FRawlings SAfalseEffect of HIV suppression on the cytokine network in blood and seminal plasma.<h4>Objective</h4>HIV infection disrupts the cytokine network and this disruption is not completely reversed by antiretroviral therapy (ART). Characterization of cytokine changes in blood and genital secretions is important for understanding HIV pathogenesis and the mechanisms of HIV sexual transmission. Here, we characterized the cytokine network in individuals longitudinally sampled before they began ART and after achieving suppression of HIV RNA.<h4>Methods</h4>We measured concentrations of 34 cytokine/chemokines using multiplex bead-based assay in blood and seminal plasma of 19 men with HIV-1 prior to and after viral suppression. We used Partial Least Squares Discriminant Analysis (PLS-DA) to visualize the difference in cytokine pattern between the time points. Any cytokines with VIP s2022-01-01T00:00:00Z2022 Apr2025-04-21T22:36:26.205Z2025-04-05T18:50:00.928ZS-EPMC89575083487309010.1097/QAD.000000000000314610.1097/qad.0000000000003146