{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Amarasekara H"],"funding":["Intramural NIH HHS","NIBIB NIH HHS","NIDDK NIH HHS","National Institute of Diabetes and Digestive and Kidney Diseases","Office of AIDS Research","National Institutes of Health","National Institute of Biomedical Imaging and Bioengineering","NIH HHS"],"pagination":["e23481"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8957522"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["113(3)"],"pubmed_abstract":["Routine patient testing for viral infections is critical to identify infected individuals for treatment and to prevent spreading of infections to others. Developing robust and reliable diagnostic tools to detect nucleic acids of viruses at the point-of-care could greatly assist the clinical management of viral infections. The remarkable stability and high binding affinity of peptide nucleic acids (PNAs) to target nucleic acids could make PNA-based biosensors an excellent starting point to develop new nucleic acid detection technologies. We report the application of cyclopentane-modified PNAs to capture target nucleic acids in a microfluidic channel, and the use of bioorthogonal PNAs conjugated to gold nanoparticles as probes to semi-quantitatively signal the presence of a target nucleic ac"],"journal":["Biopolymers"],"pubmed_title":["Cyclopentane peptide nucleic acid: Gold nanoparticle conjugates for the detection of nucleic acids in a microfluidic format."],"pmcid":["PMC8957522"],"funding_grant_id":["Z01 DK031119","ZIA DK031143","Z01 DK031143"],"pubmed_authors":["Amarasekara H","O'Farrell B","Jeans KB","Rezvan Sangsari P","Morgan NY","Appella DH","Oshaben KM"],"additional_accession":[]},"is_claimable":false,"name":"Cyclopentane peptide nucleic acid: Gold nanoparticle conjugates for the detection of nucleic acids in a microfluidic format.","description":"Routine patient testing for viral infections is critical to identify infected individuals for treatment and to prevent spreading of infections to others. Developing robust and reliable diagnostic tools to detect nucleic acids of viruses at the point-of-care could greatly assist the clinical management of viral infections. The remarkable stability and high binding affinity of peptide nucleic acids (PNAs) to target nucleic acids could make PNA-based biosensors an excellent starting point to develop new nucleic acid detection technologies. We report the application of cyclopentane-modified PNAs to capture target nucleic acids in a microfluidic channel, and the use of bioorthogonal PNAs conjugated to gold nanoparticles as probes to semi-quantitatively signal the presence of a target nucleic ac","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-18T15:20:28.073Z","creation":"2025-04-07T01:56:34.026Z"},"accession":"S-EPMC8957522","cross_references":{"pubmed":["34812507"],"doi":["10.1002/bip.23481"]}}