{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang Z"],"funding":["NIDDK NIH HHS","Medical Research Council","NIGMS NIH HHS"],"pagination":["110467"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8957708"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["38(10)"],"pubmed_abstract":["Despite their importance in tissue homeostasis and renewal, human pituitary stem cells (PSCs) are incompletely characterized. We describe a human single nucleus RNA-seq and ATAC-seq resource from pediatric, adult, and aged postmortem pituitaries (snpituitaryatlas.princeton.edu) and characterize cell-type-specific gene expression and chromatin accessibility programs for all major pituitary cell lineages. We identify uncommitted PSCs, committing progenitor cells, and sex differences. Pseudotime trajectory analysis indicates that early-life PSCs are distinct from the other age groups. Linear modeling of same-cell multiome data identifies regulatory domain accessibility sites and transcription factors that are significantly associated with gene expression in PSCs compared with other cell types"],"journal":["Cell reports"],"pubmed_title":["Single nucleus transcriptome and chromatin accessibility of postmortem human pituitaries reveal diverse stem cell regulatory mechanisms."],"pmcid":["PMC8957708"],"funding_grant_id":["R56 DK046943","MR/T012153/1","R01 GM071966","R01 DK046943"],"pubmed_authors":["Smith GR","Zamojski M","Seenarine N","Ruf-Zamojski F","Zaslavsky E","Pincas H","Troyanskaya OG","Cheng WS","Bernard DJ","Vasoya M","Yianni V","Andoniadou CL","Zhang Z","Mendelev N","Sealfon SC","Willis TL","Amper MAS","Nair VD","Turgeon JL"],"additional_accession":[]},"is_claimable":false,"name":"Single nucleus transcriptome and chromatin accessibility of postmortem human pituitaries reveal diverse stem cell regulatory mechanisms.","description":"Despite their importance in tissue homeostasis and renewal, human pituitary stem cells (PSCs) are incompletely characterized. We describe a human single nucleus RNA-seq and ATAC-seq resource from pediatric, adult, and aged postmortem pituitaries (snpituitaryatlas.princeton.edu) and characterize cell-type-specific gene expression and chromatin accessibility programs for all major pituitary cell lineages. We identify uncommitted PSCs, committing progenitor cells, and sex differences. Pseudotime trajectory analysis indicates that early-life PSCs are distinct from the other age groups. Linear modeling of same-cell multiome data identifies regulatory domain accessibility sites and transcription factors that are significantly associated with gene expression in PSCs compared with other cell types","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-05-09T21:54:50.623Z","creation":"2025-04-04T22:04:20.387Z"},"accession":"S-EPMC8957708","cross_references":{"pubmed":["35263594"],"doi":["10.1016/j.celrep.2022.110467"]}}