biostudies-literatureZhang ZNIDDK NIH HHSMedical Research CouncilNIGMS NIH HHS110467https://www.ebi.ac.uk/biostudies/studies/S-EPMC8957708biostudies-literatureUnknown38(10)Despite their importance in tissue homeostasis and renewal, human pituitary stem cells (PSCs) are incompletely characterized. We describe a human single nucleus RNA-seq and ATAC-seq resource from pediatric, adult, and aged postmortem pituitaries (snpituitaryatlas.princeton.edu) and characterize cell-type-specific gene expression and chromatin accessibility programs for all major pituitary cell lineages. We identify uncommitted PSCs, committing progenitor cells, and sex differences. Pseudotime trajectory analysis indicates that early-life PSCs are distinct from the other age groups. Linear modeling of same-cell multiome data identifies regulatory domain accessibility sites and transcription factors that are significantly associated with gene expression in PSCs compared with other cell typesCell reportsSingle nucleus transcriptome and chromatin accessibility of postmortem human pituitaries reveal diverse stem cell regulatory mechanisms.PMC8957708R56 DK046943MR/T012153/1R01 GM071966R01 DK046943Smith GRZamojski MSeenarine NRuf-Zamojski FZaslavsky EPincas HTroyanskaya OGCheng WSBernard DJVasoya MYianni VAndoniadou CLZhang ZMendelev NSealfon SCWillis TLAmper MASNair VDTurgeon JLfalseSingle nucleus transcriptome and chromatin accessibility of postmortem human pituitaries reveal diverse stem cell regulatory mechanisms.Despite their importance in tissue homeostasis and renewal, human pituitary stem cells (PSCs) are incompletely characterized. We describe a human single nucleus RNA-seq and ATAC-seq resource from pediatric, adult, and aged postmortem pituitaries (snpituitaryatlas.princeton.edu) and characterize cell-type-specific gene expression and chromatin accessibility programs for all major pituitary cell lineages. We identify uncommitted PSCs, committing progenitor cells, and sex differences. Pseudotime trajectory analysis indicates that early-life PSCs are distinct from the other age groups. Linear modeling of same-cell multiome data identifies regulatory domain accessibility sites and transcription factors that are significantly associated with gene expression in PSCs compared with other cell types2022-01-01T00:00:00Z2022 Mar2026-05-09T21:54:50.623Z2025-04-04T22:04:20.387ZS-EPMC89577083526359410.1016/j.celrep.2022.110467