{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tomar N"],"funding":["NHLBI NIH HHS","National Institutes of Health"],"pagination":["148518"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8957717"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1863(2)"],"pubmed_abstract":["The kinetics and efficiency of mitochondrial oxidative phosphorylation (OxPhos) can depend on the choice of respiratory substrates. Furthermore, potential differences in this substrate dependency among different tissues are not well-understood. Here, we determined the effects of different substrates on the kinetics and efficiency of OxPhos in isolated mitochondria from the heart and kidney cortex and outer medulla (OM) of Sprague-Dawley rats. The substrates were pyruvate+malate, glutamate+malate, palmitoyl-carnitine+malate, alpha-ketoglutarate+malate, and succinate±rotenone at saturating concentrations. The kinetics of OxPhos were interrogated by measuring mitochondrial bioenergetics under different ADP perturbations. Results show that the kinetics and efficiency of OxPhos are highly depen"],"journal":["Biochimica et biophysica acta. Bioenergetics"],"pubmed_title":["Substrate-dependent differential regulation of mitochondrial bioenergetics in the heart and kidney cortex and outer medulla."],"pmcid":["PMC8957717"],"funding_grant_id":["P01 HL116264","P01-HL116264","R01-HL151587","R01 HL151587"],"pubmed_authors":["Sadri S","Audi SH","Tomar N","Cowley AW","Dash RK","Kandel SM","Zhang X","Liang M","Yang C"],"additional_accession":[]},"is_claimable":false,"name":"Substrate-dependent differential regulation of mitochondrial bioenergetics in the heart and kidney cortex and outer medulla.","description":"The kinetics and efficiency of mitochondrial oxidative phosphorylation (OxPhos) can depend on the choice of respiratory substrates. Furthermore, potential differences in this substrate dependency among different tissues are not well-understood. Here, we determined the effects of different substrates on the kinetics and efficiency of OxPhos in isolated mitochondria from the heart and kidney cortex and outer medulla (OM) of Sprague-Dawley rats. The substrates were pyruvate+malate, glutamate+malate, palmitoyl-carnitine+malate, alpha-ketoglutarate+malate, and succinate±rotenone at saturating concentrations. The kinetics of OxPhos were interrogated by measuring mitochondrial bioenergetics under different ADP perturbations. Results show that the kinetics and efficiency of OxPhos are highly depen","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2025-04-04T12:40:47.792Z","creation":"2025-04-04T12:40:47.792Z"},"accession":"S-EPMC8957717","cross_references":{"pubmed":["34864090"],"doi":["10.1016/j.bbabio.2021.148518"]}}