biostudies-literatureTomar NNHLBI NIH HHSNational Institutes of Health148518https://www.ebi.ac.uk/biostudies/studies/S-EPMC8957717biostudies-literatureUnknown1863(2)The kinetics and efficiency of mitochondrial oxidative phosphorylation (OxPhos) can depend on the choice of respiratory substrates. Furthermore, potential differences in this substrate dependency among different tissues are not well-understood. Here, we determined the effects of different substrates on the kinetics and efficiency of OxPhos in isolated mitochondria from the heart and kidney cortex and outer medulla (OM) of Sprague-Dawley rats. The substrates were pyruvate+malate, glutamate+malate, palmitoyl-carnitine+malate, alpha-ketoglutarate+malate, and succinate±rotenone at saturating concentrations. The kinetics of OxPhos were interrogated by measuring mitochondrial bioenergetics under different ADP perturbations. Results show that the kinetics and efficiency of OxPhos are highly depenBiochimica et biophysica acta. BioenergeticsSubstrate-dependent differential regulation of mitochondrial bioenergetics in the heart and kidney cortex and outer medulla.PMC8957717P01 HL116264P01-HL116264R01-HL151587R01 HL151587Sadri SAudi SHTomar NCowley AWDash RKKandel SMZhang XLiang MYang CfalseSubstrate-dependent differential regulation of mitochondrial bioenergetics in the heart and kidney cortex and outer medulla.The kinetics and efficiency of mitochondrial oxidative phosphorylation (OxPhos) can depend on the choice of respiratory substrates. Furthermore, potential differences in this substrate dependency among different tissues are not well-understood. Here, we determined the effects of different substrates on the kinetics and efficiency of OxPhos in isolated mitochondria from the heart and kidney cortex and outer medulla (OM) of Sprague-Dawley rats. The substrates were pyruvate+malate, glutamate+malate, palmitoyl-carnitine+malate, alpha-ketoglutarate+malate, and succinate±rotenone at saturating concentrations. The kinetics of OxPhos were interrogated by measuring mitochondrial bioenergetics under different ADP perturbations. Results show that the kinetics and efficiency of OxPhos are highly depen2022-01-01T00:00:00Z2022 Feb2025-04-04T12:40:47.792Z2025-04-04T12:40:47.792ZS-EPMC89577173486409010.1016/j.bbabio.2021.148518