biostudies-literatureUnknown10Hakibilen CCellular adhesion and migration are key functions that are disrupted in numerous diseases. We report that desmin, a type-III muscle-specific intermediate filament, is a novel cell adhesion regulator. Expression of p.R406W mutant desmin, identified in patients with desmin-related myopathy, modified focal adhesion area and expression of adhesion-signaling genes in myogenic C2C12 cells. Satellite cells extracted from desmin-knock-out (DesKO) and desmin-knock-in-p.R405W (DesKI-R405W) mice were less adhesive and migrated faster than those from wild-type mice. Moreover, we observed mislocalized and aggregated vinculin, a key component of cell adhesion, in DesKO and DesKI-R405W muscles. Vinculin expression was also increased in desmin-related myopathy patient muscles. Together, our results establFrontiers in cell and developmental biology783724https://www.ebi.ac.uk/biostudies/studies/S-EPMC8957967biostudies-literatureDesmin Modulates Muscle Cell Adhesion and Migration.PMC8957967Bourgois-Rocha FCabet EDaher MTTian CAgbulut OJoanne PVicart PDelort FRivas EBatonnet-Pichon SCardoso OHakibilen CFerreiro AMadruga MLilienbaum AHenon SfalseDesmin Modulates Muscle Cell Adhesion and Migration.Cellular adhesion and migration are key functions that are disrupted in numerous diseases. We report that desmin, a type-III muscle-specific intermediate filament, is a novel cell adhesion regulator. Expression of p.R406W mutant desmin, identified in patients with desmin-related myopathy, modified focal adhesion area and expression of adhesion-signaling genes in myogenic C2C12 cells. Satellite cells extracted from desmin-knock-out (DesKO) and desmin-knock-in-p.R405W (DesKI-R405W) mice were less adhesive and migrated faster than those from wild-type mice. Moreover, we observed mislocalized and aggregated vinculin, a key component of cell adhesion, in DesKO and DesKI-R405W muscles. Vinculin expression was also increased in desmin-related myopathy patient muscles. Together, our results establ2022-01-01T00:00:00Z20222025-04-18T15:06:55.678Z2025-04-07T01:42:26.263ZS-EPMC89579673535038610.3389/fcell.2022.783724