biostudies-literatureWang LScience and Technology Commission of Shanghai MunicipalityNational Key Research and Development Program of China Stem Cell and Translational ResearchNational Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaShanghai Jiao Tong University149-161https://www.ebi.ac.uk/biostudies/studies/S-EPMC8958472biostudies-literatureUnknown16Electrospun fibers, with proven ability to promote tissue regeneration, are widely being explored for rotator cuff repairing. However, without post treatment, the microstructure of the electrospun scaffold is vastly different from that of natural extracellular matrix (ECM). Moreover, during mechanical loading, the nanofibers slip that hampers the proliferation and differentiation of migrating stem cells. Here, electrospun nanofiber scaffolds, with crimped nanofibers and welded joints to biomimic the intricate natural microstructure of tendon-to-bone insertion, were prepared using poly(ester-urethane)urea and gelatin via electrospinning and double crosslinking by a multi-bonding network densification strategy. The crimped nanofiber scaffold (CNS) features bionic tensile stress and induces cBioactive materialsCrimped nanofiber scaffold mimicking tendon-to-bone interface for fatty-infiltrated massive rotator cuff repair.PMC8958472194419017011944190170031972923816719202018YFC11062012018YFC110620218441902800817723418187175319441901702819021862018YFC1106200Jiang JZhang JDu JGao HChen SKang YZhao JZhu TWang LfalseCrimped nanofiber scaffold mimicking tendon-to-bone interface for fatty-infiltrated massive rotator cuff repair.Electrospun fibers, with proven ability to promote tissue regeneration, are widely being explored for rotator cuff repairing. However, without post treatment, the microstructure of the electrospun scaffold is vastly different from that of natural extracellular matrix (ECM). Moreover, during mechanical loading, the nanofibers slip that hampers the proliferation and differentiation of migrating stem cells. Here, electrospun nanofiber scaffolds, with crimped nanofibers and welded joints to biomimic the intricate natural microstructure of tendon-to-bone insertion, were prepared using poly(ester-urethane)urea and gelatin via electrospinning and double crosslinking by a multi-bonding network densification strategy. The crimped nanofiber scaffold (CNS) features bionic tensile stress and induces c2022-01-01T00:00:00Z2022 Oct2025-04-04T21:33:21.959Z2025-04-04T21:33:21.959ZS-EPMC89584723538632910.1016/j.bioactmat.2022.01.031