biostudies-literatureLi HNIDCR NIH HHSNIAID NIH HHSNational Institutes of Health3303-3313https://www.ebi.ac.uk/biostudies/studies/S-EPMC8958665biostudies-literatureUnknown7(12)Multidrug-resistant bacterial infections have become a global threat. We recently disclosed that the known IKK-β inhibitor IMD-0354 and subsequent analogues abrogate colistin resistance in several Gram-negative strains. Herein, we report the activity of a second-generation library of IMD-0354 analogues incorporating a benzimidazole moiety as an amide isostere. We identified several analogues that show increased colistin potentiation activity against Gram-negative bacteria.ACS infectious diseasesBenzimidazole Isosteres of Salicylanilides Are Highly Active Colistin Adjuvants.PMC8958665R01 AI136904R01 AI104895RO1AI136904RO1AI104895R01 DE022350Li HMelander RJErnst RKSmith RJania LAKoller BHMelander CMattingly AEfalseBenzimidazole Isosteres of Salicylanilides Are Highly Active Colistin Adjuvants.Multidrug-resistant bacterial infections have become a global threat. We recently disclosed that the known IKK-β inhibitor IMD-0354 and subsequent analogues abrogate colistin resistance in several Gram-negative strains. Herein, we report the activity of a second-generation library of IMD-0354 analogues incorporating a benzimidazole moiety as an amide isostere. We identified several analogues that show increased colistin potentiation activity against Gram-negative bacteria.2021-01-01T00:00:00Z2021 Dec2025-04-05T12:52:53.361Z2025-04-05T12:52:53.361ZS-EPMC89586653475205510.1021/acsinfecdis.1c00463