biostudies-literatureTran HTTMinistry of Science and TechnologyUK Research and InnovationBiotechnology and Biological Sciences Research Council607-609https://www.ebi.ac.uk/biostudies/studies/S-EPMC8959334biostudies-literatureUnknown8(2)<h4>Background</h4>African swine fever (ASF) is a highly contagious and deadly viral disease affecting domestic and wild pigs of all ages. African swine fever virus (ASFV) has spread rapidly through Eastern and Southeastern Asia first appearing in Vietnam in 2019.<h4>Objectives</h4>Molecular typing of African swine fever virus (ASFV) in Vietnam has identified two principal variants circulating based on the sequencing of the intergenic region (IRG) between the I73R and I329L genes. Identification of additional genetic markers would enable higher resolution tracing of outbreaks within the country.<h4>Methods</h4>Sequence analysis suggested the IRG between the A179L and A137R genes may also exhibit variability, PCR primers were designed and samples from Vietnam were subject to Sanger sequenciVeterinary medicine and scienceNovel method for sub-grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genes.PMC8959334BBS/E/I/00007037BBS/E/I/00007039BBS/E/I/00007035ĐTĐL.CN‐75/19Van Hoang TLy DVNetherton CLDang HVChu NTTruong ADNguyen HTTran HTTDang AKfalseNovel method for sub-grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genes.<h4>Background</h4>African swine fever (ASF) is a highly contagious and deadly viral disease affecting domestic and wild pigs of all ages. African swine fever virus (ASFV) has spread rapidly through Eastern and Southeastern Asia first appearing in Vietnam in 2019.<h4>Objectives</h4>Molecular typing of African swine fever virus (ASFV) in Vietnam has identified two principal variants circulating based on the sequencing of the intergenic region (IRG) between the I73R and I329L genes. Identification of additional genetic markers would enable higher resolution tracing of outbreaks within the country.<h4>Methods</h4>Sequence analysis suggested the IRG between the A179L and A137R genes may also exhibit variability, PCR primers were designed and samples from Vietnam were subject to Sanger sequenci2022-01-01T00:00:00Z2022 Mar2026-06-18T05:05:03.825Z2025-06-01T01:29:28.902ZS-EPMC89593343496713310.1002/vms3.702