{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Schultz M"],"pubmed_abstract":["Septic arthritis, most often caused by <i>Staphylococcus aureus</i>, is a rapidly progressive and destructive joint disease with substantial mortality and morbidity. <i>Staphylococcus aureus</i> lipoproteins (Lpps) are known to induce arthritis and bone destruction. Here, we aimed to investigate the bone resorptive effect of <i>S. aureus</i> Lpps in a murine arthritis model by intra-articular injection of purified <i>S. aureus</i> Lpps, synthetic lipopeptides, and live <i>S. aureus</i> strains. Analyses of the bone mineral density (BMD) of the distal femur bone were performed. Intra-articular injection of both live <i>S. aureus</i> and purified <i>S. aureus</i> Lpps were shown to significantly decrease total- and trabecular BMD. Liquid chromatography-mass spectrometry analyses revealed that the Lpps expressed by <i>S. aureus</i> SA113 strain contain both diacyl and triacyl lipid moieties. Interestingly, synthetic diacylated lipopeptide, Pam<sub>2</sub>CSK<sub>4</sub>, was more potent in inducing bone resorption than synthetic triacylated lipopeptide, Pam<sub>3</sub>CSK<sub>4</sub>. Modified lipoproteins lacking the lipid moiety were deprived of their bone resorptive abilities. Monocyte depletion by clodronate liposomes fully abrogated the bone resorptive capacity of <i>S. aureus</i> lipoproteins. Our data suggest that <i>S. aureus</i> Lpps induce bone resorption in locally-induced murine arthritis, an effect mediated by their lipid-moiety through monocytes/macrophages."],"journal":["Frontiers in microbiology"],"pagination":["843799"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8959583"],"repository":["biostudies-literature"],"pubmed_title":["Lipoproteins Cause Bone Resorption in a Mouse Model of <i>Staphylococcus aureus</i> Septic Arthritis."],"pmcid":["PMC8959583"],"pubmed_authors":["Jarneborn A","Wienken CM","Froning M","Hu Z","Jin T","Schultz M","Nguyen MT","Ali A","Pullerits R","Mohammad M","Hayen H","Gotz F"],"additional_accession":[]},"is_claimable":false,"name":"Lipoproteins Cause Bone Resorption in a Mouse Model of <i>Staphylococcus aureus</i> Septic Arthritis.","description":"Septic arthritis, most often caused by <i>Staphylococcus aureus</i>, is a rapidly progressive and destructive joint disease with substantial mortality and morbidity. <i>Staphylococcus aureus</i> lipoproteins (Lpps) are known to induce arthritis and bone destruction. Here, we aimed to investigate the bone resorptive effect of <i>S. aureus</i> Lpps in a murine arthritis model by intra-articular injection of purified <i>S. aureus</i> Lpps, synthetic lipopeptides, and live <i>S. aureus</i> strains. Analyses of the bone mineral density (BMD) of the distal femur bone were performed. Intra-articular injection of both live <i>S. aureus</i> and purified <i>S. aureus</i> Lpps were shown to significantly decrease total- and trabecular BMD. Liquid chromatography-mass spectrometry analyses revealed that the Lpps expressed by <i>S. aureus</i> SA113 strain contain both diacyl and triacyl lipid moieties. Interestingly, synthetic diacylated lipopeptide, Pam<sub>2</sub>CSK<sub>4</sub>, was more potent in inducing bone resorption than synthetic triacylated lipopeptide, Pam<sub>3</sub>CSK<sub>4</sub>. Modified lipoproteins lacking the lipid moiety were deprived of their bone resorptive abilities. Monocyte depletion by clodronate liposomes fully abrogated the bone resorptive capacity of <i>S. aureus</i> lipoproteins. Our data suggest that <i>S. aureus</i> Lpps induce bone resorption in locally-induced murine arthritis, an effect mediated by their lipid-moiety through monocytes/macrophages.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-19T20:10:26.523Z","creation":"2025-04-19T20:10:26.523Z"},"accession":"S-EPMC8959583","cross_references":{"pubmed":["35356518"],"doi":["10.3389/fmicb.2022.843799"]}}