{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Okada M"],"funding":["Johns Hopkins Malaria Research Institute","Congressionally Directed Medical Research Programs","Pew Charitable Trusts","National Institute of Diabetes and Digestive and Kidney Diseases","NIAID NIH HHS","National Institutes of Health","National Institute of General Medical Sciences","National Institute of Allergy and Infectious Diseases","NIDDK NIH HHS","Burroughs Wellcome Fund","Bloomberg Philanthropies","NIH HHS","NIGMS NIH HHS"],"pagination":["e73208"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8959605"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11"],"pubmed_abstract":["Isopentenyl pyrophosphate (IPP) is an essential metabolic output of the apicoplast organelle in <i>Plasmodium falciparum</i> malaria parasites and is required for prenylation-dependent vesicular trafficking and other cellular processes. We have elucidated a critical and previously uncharacterized role for IPP in apicoplast biogenesis. Inhibiting IPP synthesis blocks apicoplast elongation and inheritance by daughter merozoites, and apicoplast biogenesis is rescued by exogenous IPP and polyprenols. Knockout of the only known isoprenoid-dependent apicoplast pathway, tRNA prenylation by MiaA, has no effect on blood-stage parasites and thus cannot explain apicoplast reliance on IPP. However, we have localized an annotated polyprenyl synthase (PPS) to the apicoplast. PPS knockdown is lethal to p"],"journal":["eLife"],"pubmed_title":["Critical role for isoprenoids in apicoplast biogenesis by malaria parasites."],"pmcid":["PMC8959605"],"funding_grant_id":["T32 AI007417","W81XWH1810060","R35 GM133764","U54 DK110858","T32DK007115","1S10OD021505","1S10OD016232","U54DK110858","1S10OD018210","R01AI125534","S10 OD016232","S10 OD018210","32099","R01 AI125534","S10 OD021505","T32 DK007115","R35GM133764","T32AI007417","1011969"],"pubmed_authors":["Maschek JA","Okada M","Mixon A","Prigge ST","Swift RP","Sigala PA","Rajaram K"],"additional_accession":[]},"is_claimable":false,"name":"Critical role for isoprenoids in apicoplast biogenesis by malaria parasites.","description":"Isopentenyl pyrophosphate (IPP) is an essential metabolic output of the apicoplast organelle in <i>Plasmodium falciparum</i> malaria parasites and is required for prenylation-dependent vesicular trafficking and other cellular processes. We have elucidated a critical and previously uncharacterized role for IPP in apicoplast biogenesis. Inhibiting IPP synthesis blocks apicoplast elongation and inheritance by daughter merozoites, and apicoplast biogenesis is rescued by exogenous IPP and polyprenols. Knockout of the only known isoprenoid-dependent apicoplast pathway, tRNA prenylation by MiaA, has no effect on blood-stage parasites and thus cannot explain apicoplast reliance on IPP. However, we have localized an annotated polyprenyl synthase (PPS) to the apicoplast. PPS knockdown is lethal to p","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-05-31T06:03:13.189Z","creation":"2025-02-19T01:55:03.882Z"},"accession":"S-EPMC8959605","cross_references":{"pubmed":["35257658"],"doi":["10.7554/eLife.73208"]}}