biostudies-literatureUnknown9Zhao YPreviously, a new copper-zinc SOD (CuZnSOD) isolated from chestnut rose (<i>Rosa roxburghii</i>) with good stability was described. In this study, the biosynthetic approach was used to create recombinant CuZnSOD. RACE PCR was also used to amplify the full-length CuZnSOD gene from chestnut rose, and the ORF segment was expressed in <i>E. coli</i> BL21 and <i>P. pastoris</i> GS115. For characterization, the enzyme was isolated in two steps in <i>E. coli</i> and one step in <i>P. pastoris</i>. The biochemical properties of the two recombinant enzymes were similar, and their optimal reaction pH and temperature were 6.0 and 50°C, respectively. According to molecular dynamics simulation, the CuZnSOD showed high stability from 70 to 90°C, and eight amino acids are important for enzyme thermal staFrontiers in nutrition850824https://www.ebi.ac.uk/biostudies/studies/S-EPMC8959677biostudies-literatureProduction of a Novel Superoxide Dismutase by <i>Escherichia coli</i> and <i>Pichia pastoris</i> and Analysis of the Thermal Stability of the Enzyme.PMC8959677Zhang WZhao LLiao XWang YRao LZhao YfalseProduction of a Novel Superoxide Dismutase by <i>Escherichia coli</i> and <i>Pichia pastoris</i> and Analysis of the Thermal Stability of the Enzyme.Previously, a new copper-zinc SOD (CuZnSOD) isolated from chestnut rose (<i>Rosa roxburghii</i>) with good stability was described. In this study, the biosynthetic approach was used to create recombinant CuZnSOD. RACE PCR was also used to amplify the full-length CuZnSOD gene from chestnut rose, and the ORF segment was expressed in <i>E. coli</i> BL21 and <i>P. pastoris</i> GS115. For characterization, the enzyme was isolated in two steps in <i>E. coli</i> and one step in <i>P. pastoris</i>. The biochemical properties of the two recombinant enzymes were similar, and their optimal reaction pH and temperature were 6.0 and 50°C, respectively. According to molecular dynamics simulation, the CuZnSOD showed high stability from 70 to 90°C, and eight amino acids are important for enzyme thermal sta2022-01-01T00:00:00Z20222026-05-30T20:57:41.494Z2025-04-04T10:02:21.661ZS-EPMC89596773535673610.3389/fnut.2022.850824