{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9"],"submitter":["Fagbadebo FO"],"pubmed_abstract":["The mitochondrial outer membrane (MOM)-anchored GTPase Miro1, is a central player in mitochondrial transport and homeostasis. The dysregulation of Miro1 in amyotrophic lateral sclerosis (ALS) and Parkinson's disease (PD) suggests that Miro1 may be a potential biomarker or drug target in neuronal disorders. However, the molecular functionality of Miro1 under (patho-) physiological conditions is poorly known. For a more comprehensive understanding of the molecular functions of Miro1, we have developed Miro1-specific nanobodies (Nbs) as novel research tools. We identified seven Nbs that bind either the N- or C-terminal GTPase domain of Miro1 and demonstrate their application as research tools for proteomic and imaging approaches. To visualize the dynamics of Miro1 in real time, we selected in"],"journal":["Frontiers in molecular biosciences"],"pagination":["835302"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8960383"],"repository":["biostudies-literature"],"pubmed_title":["A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1."],"pmcid":["PMC8960383"],"pubmed_authors":["Jarjour G","Zittlau K","Bartlick N","Froehlich T","Nueske S","Scholz A","Rothbauer U","Kaiser PD","Fagbadebo FO","Wagner TR","Traenkle B","Macek B"],"additional_accession":[]},"is_claimable":false,"name":"A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1.","description":"The mitochondrial outer membrane (MOM)-anchored GTPase Miro1, is a central player in mitochondrial transport and homeostasis. The dysregulation of Miro1 in amyotrophic lateral sclerosis (ALS) and Parkinson's disease (PD) suggests that Miro1 may be a potential biomarker or drug target in neuronal disorders. However, the molecular functionality of Miro1 under (patho-) physiological conditions is poorly known. For a more comprehensive understanding of the molecular functions of Miro1, we have developed Miro1-specific nanobodies (Nbs) as novel research tools. We identified seven Nbs that bind either the N- or C-terminal GTPase domain of Miro1 and demonstrate their application as research tools for proteomic and imaging approaches. To visualize the dynamics of Miro1 in real time, we selected in","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-04-08T17:57:17.01Z","creation":"2025-04-06T21:53:57.959Z"},"accession":"S-EPMC8960383","cross_references":{"pubmed":["35359597"],"doi":["10.3389/fmolb.2022.835302"]}}