biostudies-literatureUnknown9Fagbadebo FOThe mitochondrial outer membrane (MOM)-anchored GTPase Miro1, is a central player in mitochondrial transport and homeostasis. The dysregulation of Miro1 in amyotrophic lateral sclerosis (ALS) and Parkinson's disease (PD) suggests that Miro1 may be a potential biomarker or drug target in neuronal disorders. However, the molecular functionality of Miro1 under (patho-) physiological conditions is poorly known. For a more comprehensive understanding of the molecular functions of Miro1, we have developed Miro1-specific nanobodies (Nbs) as novel research tools. We identified seven Nbs that bind either the N- or C-terminal GTPase domain of Miro1 and demonstrate their application as research tools for proteomic and imaging approaches. To visualize the dynamics of Miro1 in real time, we selected inFrontiers in molecular biosciences835302https://www.ebi.ac.uk/biostudies/studies/S-EPMC8960383biostudies-literatureA Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1.PMC8960383Jarjour GZittlau KBartlick NFroehlich TNueske SScholz ARothbauer UKaiser PDFagbadebo FOWagner TRTraenkle BMacek BfalseA Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1.The mitochondrial outer membrane (MOM)-anchored GTPase Miro1, is a central player in mitochondrial transport and homeostasis. The dysregulation of Miro1 in amyotrophic lateral sclerosis (ALS) and Parkinson's disease (PD) suggests that Miro1 may be a potential biomarker or drug target in neuronal disorders. However, the molecular functionality of Miro1 under (patho-) physiological conditions is poorly known. For a more comprehensive understanding of the molecular functions of Miro1, we have developed Miro1-specific nanobodies (Nbs) as novel research tools. We identified seven Nbs that bind either the N- or C-terminal GTPase domain of Miro1 and demonstrate their application as research tools for proteomic and imaging approaches. To visualize the dynamics of Miro1 in real time, we selected in2022-01-01T00:00:00Z20222026-04-08T17:57:17.01Z2025-04-06T21:53:57.959ZS-EPMC89603833535959710.3389/fmolb.2022.835302