biostudies-literatureUnknown9Xu STo study the effects of energy and dietary fiber on breast development in gilts and its possible mechanisms, 32 gilts (Landrace × Yorkshire) were randomly allocated into a 2 × 2 factorial design to receive a diet with low or high energy [LE: 33.37 MJ/d digestible energy (DE); HE: 41.87 MJ/d DE] and low or high fiber (LF: 0.3 kg/d dietary fiber, HF: 0.6 kg/d dietary fiber). The weight of breast tissue was recorded. The mammary glands were collected for further analyses. The high energy intake increased the relative weight of breast tissue (<i>p</i> < 0.05) and the content of breast fat (<i>p</i> < 0.05). At the same time, the oil red staining of breast slices also showed an increase in breast fat content in high-energy treatment. High energy intake increased the DNA concentration in breast Frontiers in veterinary science830392https://www.ebi.ac.uk/biostudies/studies/S-EPMC8960423biostudies-literatureEffects of Energy and Dietary Fiber on the Breast Development in Gilt.PMC8960423Cao MLi JZhuo YLin YXu HChe LFeng BWang JTang LYang YFang ZJiang XXu SChen SWufalseEffects of Energy and Dietary Fiber on the Breast Development in Gilt.To study the effects of energy and dietary fiber on breast development in gilts and its possible mechanisms, 32 gilts (Landrace × Yorkshire) were randomly allocated into a 2 × 2 factorial design to receive a diet with low or high energy [LE: 33.37 MJ/d digestible energy (DE); HE: 41.87 MJ/d DE] and low or high fiber (LF: 0.3 kg/d dietary fiber, HF: 0.6 kg/d dietary fiber). The weight of breast tissue was recorded. The mammary glands were collected for further analyses. The high energy intake increased the relative weight of breast tissue (<i>p</i> < 0.05) and the content of breast fat (<i>p</i> < 0.05). At the same time, the oil red staining of breast slices also showed an increase in breast fat content in high-energy treatment. High energy intake increased the DNA concentration in breast 2022-01-01T00:00:00Z20222026-04-08T17:29:23.885Z2025-04-04T10:02:31.987ZS-EPMC89604233535967110.3389/fvets.2022.830392